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Filtration

Filtration

Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...

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Updated: Jun 18, 2025

Estimating Virus Production Rates in Aquatic Systems

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

优化病毒过用于连续处理,使用高面积比率的连续过.

Sal Giglia¹, Ben Cacace¹, Jacob McCoskey¹

¹MilliporeSigma, Bedford, Massachusetts, USA.

Biotechnology and bioengineering

|July 31, 2024

概括

此摘要是机器生成的。

连续生物处理采用连续病毒过,过面积比例高,以克服低流量挑战. 这种新的方法提高了病毒去除效率,并延长了生物制药制造中的过器寿命.

关键词:

连续加工是连续加工的过程.病毒清除时间病毒过器病毒过器病毒过,病毒过.

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A Small Volume Procedure for Viral Concentration from Water

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Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

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Published on: March 17, 2023

相关实验视频

Last Updated: Jun 18, 2025

Estimating Virus Production Rates in Aquatic Systems

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

A Small Volume Procedure for Viral Concentration from Water

A Small Volume Procedure for Viral Concentration from Water

Published on: February 3, 2015

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Published on: March 17, 2023

科学领域:

生物制药制造业生物制药制造业
工艺工程的过程工程.
过技术过技术的使用

背景情况:

连续生物处理在批量操作上具有优势,但在集成病毒过等传统步骤方面面临挑战.
持续病毒过中的低流量条件可能会损害病毒保留性能,阻碍采用.
现有的方法在持续流系统中难以保持高病毒清除率.

研究的目的:

开发和评估一种用于连续生物处理的新型连续病毒过方法.
在连续运行条件下实现目标病毒保留水平.
评估过器面积比对性能和寿命的影响.

主要方法:

病毒过器在连续配置中连续运行200小时.
在第一阶段和第二阶段过器之间使用了高面积比率 (13:1).
在第一阶段5L/m2/h的流量下运行,挑战了典型的连续过参数.

主要成果:

在连续过列车中实现了小鼠病毒 (MVM) 记录减少值 (LRV) ≥6.7.
第一个阶段的过器提供了~4LRV,而第二个阶段的过液显示没有可检测的病毒.
第一个阶段的过器控制了污染和过器列车的寿命,通过串行设置证明了有效的保护.

结论:

具有高面积比率的连续病毒过是一种可行的策略,可以在连续生物处理中增强病毒清除.
这种方法克服了低流量操作的局限性,并提高了整体工艺效率和安全性.
这些发现为优化生物制造中的连续病毒过列车提供了关键的见解.