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Updated: Jun 18, 2025

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
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Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

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塑纳米粒子辅助单分子动态结合蛋白激酶活性数字计数数码计数

Qingnan Li1,2, Wenzhi Qiang2, Yanan Deng1

  • 1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. lehuixiao@csu.edu.cn.

Chemical communications (Cambridge, England)
|July 31, 2024
PubMed
概括

我们使用等离子纳米粒子开发了一种用于蛋白激酶活性的新型数字计数试验. 这种方法提高了单分子检测的灵敏度和选择性,使低水平检测能够用于药物查中的应用.

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An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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