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瘤微环境的变化标志着从血清性边界瘤过渡到低度血清性癌瘤
Rodrigo Vallejos1,2, Almira Zhantuyakova2,3, Gian Luca Negri4
1Department of Genome Sciences and Technology, University of British Columbia, Vancouver, BC, Canada.
The Journal of pathology
|July 31, 2024
概括
低度血清性卵巢癌 (LGSC) 与其前身血清性边缘性瘤 (SBTs) 相比,呈现出明显的瘤微环境变化. 针对这些微环境差异,特别是FAP+流层,可能为LGSC提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 蛋白质组学是指蛋白质组学.
- 免疫学 免疫学 免疫学
背景情况:
- 低度血清性卵巢癌 (LGSC) 是一种罕见的,致命的卵巢癌亚型,与高度血清性卵巢癌 (HGSC) 不同.
- LGSC起源于血清边缘性卵巢瘤 (SBTs),但转化机制尚不清楚.
- 了解LGSC生物学需要研究其与前体病变和瘤微环境的关系.
研究的目的:
- 为了比较LGSC,HGSC和SBTs的蛋白质组概况.
- 为了确定LGSC及其前体SBT之间的瘤微环境的差异.
- 在LGSC瘤微环境中探索潜在的治疗点.
主要方法:
- 来自LGSC (n=11),HGSC (n=19) 和SBTs (n=26) 的甲固定,嵌组织的整体蛋白质组概况.
- 差异性蛋白质丰度分析,以区分瘤组织型.
- 多重免疫组织化学 (IHC) 针对瘤层中的免疫细胞标记物 (CD20,CD79a,CD3,CD8,CD68).
主要成果:
- 蛋白质组分析成功地区分了卵巢上皮质瘤组织型.
- 与瘤微环境相关的蛋白质在LGSC和SBTs之间表达不同.
- 纤维细胞激活蛋白 (FAP) 是LGSC与SBT中差异性最丰富的蛋白质.
- 与SBTs相比,LGSC FAP+流体表现出调节性T细胞 (Tregs) 和M2巨细胞的增加.
结论:
- 在从SBT转变为LGSC的过程中,瘤微环境发生显著变化.
- 由FAP+流体,Tregs和M2巨细胞特征的LGSC瘤微环境,支持瘤发生和进展.
- 向瘤微环境是LGSC的一个有前途的治疗策略.
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