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再组合的阿尔法疹病毒的免疫特征,其中一个包裹嵌入的环状病毒蛋白质Cap

Chenhe Lu¹, Haimin Li¹, Wenjing Chen¹

¹MOA Key Laboratory of Animal Virology, Zhejiang University Center for Veterinary Sciences, Hangzhou, China.

Frontiers in immunology

|July 31, 2024

概括

这项研究开发了一种新型重组伪狂犬病病毒 (PRV) 疫苗,该疫苗表达了猪圈病毒2 (PCV2) 帽子蛋白. PRV-PCV2候选疫苗提供了对PRV和PCV2挑战的完整保护.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
基因工程是一种基因工程.

背景情况:

伪狂热病毒 (PRV) 是一种新兴的动物性病原体,具有适合重组疫苗开发的大型基因组.
目前的重组PRV疫苗独立表达异基因,限制它们的表面显示和免疫性.
在病毒表面组装外来抗原对于提高疫苗疗效至关重要.

研究的目的:

构建一个重组PRV (PRV-Cap),在它的表面上显示猪圈病毒2 (PCV2) 帽子蛋白.
评估PRV-Cap对PRV和PCV2感染的免疫性和保护功效.
阐明疫苗保护作用背后的免疫机制.

主要方法:

利用Cre-loxP和CRISPR-Cas9系统将PCV2 Cap基因插入PRV gE基因的细胞外域,删除gE/TK基因.
评估了与父病毒相比,复合PRV (PRV-Cap) 的复制能力.
在小鼠中进行免疫性测定,包括中和抗体测试,ELISPOT和流细胞计,以分析T和B细胞反应.

主要成果:

PRV-Cap的复制能力与父母的PRV相似.
用PRV-Cap免疫的小鼠对致命的PRV和PCV2挑战表现出100%的抵抗力.
接种PRV-Cap疫苗诱导了PRV特异性中和抗体,PCV2特异性T细胞反应 (IFN-γ) 和强大的CD4+ Tfh细胞依赖B细胞激活,产生有效的记忆T和B细胞.

结论:

在其表面显示PCV2 Cap蛋白的重组PRV是一种有前途的疫苗候选人,可以获得对PRV和PCV2的联合免疫力.
这种方法为开发针对PRV和PCV2.2的双价疫苗提供了一个具有成本效益的策略.
这项研究提供了结合使用工程病毒载体的联合疫苗策略的免疫机制的见解.

关键词:

在IFN-γ中使用IFN.仿真伪狂犬病病毒仿真伪狂犬病病毒这种病毒是Circovirus.免疫力是一种免疫力.记忆的反应,记忆的反应.

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