相关实验视频

Updated: Jun 18, 2025

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.4K

多氨基-tRNA合成酶复合体的形成限制了乱伦的tRNA校对

Rylan R Watkins1, Anna Vradi1, Irina Shulgina1

  • 1Department of Chemistry and Biochemistry, Center for RNA Biology, Ohio State University, Columbus, OH, United States.

Frontiers in microbiology
|July 31, 2024
PubMed
概括

杆菌使用独特的脱糖酶来纠正蛋白质合成中的错误,特别是从proline tRNA中去除错误充电的氨酸. 这些发现揭示了对转化忠实性和潜在药物点的新见解.

关键词:
在Trypanosoma brucei的研究中.氨基酸-tRNA合成酶.多氨基-tRNA合成酶复合体这是一个tRNARNA.转编辑 转编辑翻译翻译翻译翻译翻译翻译

更多相关视频

High-throughput Gene Tagging in Trypanosoma brucei11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

7.9K
Examination of the Telomere G-overhang Structure in Trypanosoma brucei15:25

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Published on: January 26, 2011

14.3K

相关概念视频

Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
9.7K
tRNA Activation02:26

tRNA Activation

Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
19.2K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
11.9K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Proofreading01:43

Proofreading

Overview
53.9K