BlpU是Streptococcus thermophilus中的一个广谱细菌素
John A Renye1, George A Somkuti1, Phoebe X Qi1
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agricultural, Wyndmoor, PA, United States.
Frontiers in microbiology
|July 31, 2024
概括
这项研究将Streptococcus thermophilus B59671中的BlpU确定为一种广谱细菌素. 其高表达率有助于该菌株独特的抗菌活性,而热素13也表现出广泛的抗菌作用.
科学领域:
- 微生物学 微生物学
- 细菌因子是一种细菌因子.
- 食品科学 食品科学 食品科学
背景情况:
- 热杆菌菌菌株B59671产生细菌菌素热菌素110和热菌素13.
- 细菌样 (blp) 基因集群与细菌生产有关.
研究的目的:
- 研究blpU和blpK基因在S. thermophilus B59671.1.的细菌素生产中的作用.
- 描述BlpU和热爱素的抗菌活性和光谱 13.
主要方法:
- 基因删除突变 (blpK,blpC) 在S. thermophilus B59671.1.中被创造出来.
- BlpU是通过大肠杆菌的过度表达和化学合成产生的.
- 对各种细菌菌株进行了抗菌活性测试.
- 基因转录水平 (blpU) 在菌株之间进行了比较.
主要成果:
- 删除blpK并没有取消抗菌活性.
- 成熟的BlpU表现出针对S. thermophilus,S. mutans,P. acidilactici和L. innocua的广泛的抗菌活性.
- 与S. thermophilus LMD-9.9相比,B59671中的blpU转录显著更高.
- 一种blpC删除突变体显示出抗菌活性,这表明热素13的作用.
结论:
- BlpU是一种功能性,广谱的细菌素,不需要翻译后修饰才能发挥活性.
- 在B59671中高的blpU表达可能有助于其独特的抗菌特征.
- 热素13表现出物种内和物种间的抗菌活性.
相关概念视频
00:57Antibiotic Selection
49.1K
Overview
49.1K
01:28Biological Methods for Microbial Control
1.4K
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
1.4K
01:30Bacterial Phylum Actinobacteria
869
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
869
01:28Anoxygenic Phototrophic Bacteria
1.3K
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
1.3K
01:29Microbes in Food Production
409
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
409
01:25Inhibitors of Bacterial Protein Synthesis
127
Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
127