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对于的量化方法是独立的,不管真菌的形态如何

Amanda B Soares¹, Maria C de Albuquerque¹, Leticia M Rosa¹

¹Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP) Araraquara, Araraquara, São Paulo Brazil.

Microbial cell (Graz, Austria)

|July 31, 2024

概括

鉴定Candida albicans的数量是具有挑战性的,因为它的形状变化. 这项研究发现,常见的方法,如细胞计数,殖民地形成单位 (CFU) 和可活性PCR (vPCR),无论真菌形式如何,都显示出不良一致性和缺乏线性关系.

科学领域:

微生物学微生物学
菌类学菌类学是指菌类学.
定量生物学定量生物学

背景情况:

虫 Candida albicans 呈现多态性,在酵母和丝状形式之间切换.
这种形态可塑性可以在微生物量化方法中引入偏差.
准确的量化对于了解C. albicans的发病和治疗疗效至关重要.

研究的目的:

为了比较Candida albicans在各种形态 (酵母,细丝,生物膜) 的不同量化方法的一致性和相关性.
评估C. albicans形态对常见微生物量化技术可靠性的影响.
根据真菌形态和生长条件,确定最合适的量化方法.

主要方法:

细胞/毫升,殖民地形成单位 (CFU) /毫升和生命力聚合酶链反应 (vPCR) 的比较,用于量化三种C. albicans菌株.
使用生物膜XTT测定对代谢活动的评估.
统计分析包括协议分析 (ICC,CCC,布兰德-阿尔特曼地块) 和皮尔森相关性.
主要组件分析 (PCA) 用于可视化方法相似性.

主要成果:

在所有测试的量化方法之间,无论真菌形态如何,都观察到显著缺乏一致性.
方法之间发现了强烈的相关性,但没有一致的线性关系,除了特定的酵母和生物膜比较.
活性PCR (vPCR) 倾向于高估细胞数量,而CFU/mL与直接细胞计数相比低估了它们.
主要组件分析揭示了不同的方法集群,XTT检测是生物膜中最不相似的.

结论:

对于Candida albicans的标准微生物量化方法表现出差的一致性,并且缺乏跨不同形态的线性关系.
CFU/mL低估了细胞计数,vPCR高估了细胞计数,突出了固有的偏差.
选择量化方法显著影响结果,需要仔细考虑真菌形态和生长状态.

关键词:

在Candida albicans中使用.细胞计数细胞计数的数量.殖民地计数数目殖民地计数形形 (hyphae) 是一种形.这是微生物的微生物.聚合酶连锁反应的发生.酵母酵母是一种酵母.

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