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通过NGS进行ICU护理 - 基于感染病原体的鉴定:一项比较研究

Aishwariya Ramanathan¹, Anusuya Marimuthu¹, Lalanika M Abayasekara²

¹Micro Genomics India Pvt Ltd, 16, Wallace Garden First street, Thousand lights, Chennai, 600006, India.

|July 31, 2024

概括

下一代测序分析Bactfast®和Fungifast®显示出在重症监护室 (ICU) 诊断败血症的前景. Bactfast®表现出高灵敏度和特异性,有可能改善严重败血症病例患者的治疗结果.

科学领域:

临床微生物学和传染病.
用于病原体识别的基因组技术.
重症监护室 (ICU) 诊断和患者管理.

背景情况:

败血症是导致死亡的主要原因,占全球死亡人数的20%,占ICU入院人数的很大一部分.
精确和快速诊断导致性败血症的病原体是具有挑战性的,因为多微生物感染,共生和污染物.
像培养和PCR这样的传统方法在克服这些诊断复杂性方面存在局限性.

研究的目的:

评估Bactfast®和Fungifast®测定作为治疗不治愈的ICU败血症的先进诊断工具的有效性.
评估基于下一代测序 (NGS) 的测试对真正的病原体与共生物和污染物的区分的能力.
在现实ICU环境中确定Bactfast®和Fungifast®的诊断性能.

主要方法:

一项回顾性研究在印度奈的四个ICU中进行.
在中央NGS设施使用Bactfast®和Fungifast®试验处理样品.
结果与传统的微生物学测试和多学科团队的临床数据相关联.

主要成果:

Bactfast®试验获得了94.1%的灵敏度和86.6%的特异性,超过了之前的数据.
Bactfast®与临床确认呈正相关性,表明可靠的病原体鉴定.
Fungifast®的特异性很高 (99.4%),但由于阳性真菌培养数量很少,需要进一步研究.

结论:

Bactfast®提供了无偏见的病原体识别,有可能减少危及生命的ICU败血症的死亡率和发病率.
基于NGS的方法有效地将病原体与污染物和共生物区分开来.
需要使用多种方法和更大的样本大小对Fungifast®进行进一步的研究,以充分评估其诊断灵敏度.

关键词:

16s rRNA 序列的测序方法 ITS 测序的排序方式转基因组学是指转基因组学.下一代测序测序是什么泛细菌鉴定细菌鉴定泛真菌识别方法泛真菌识别方法败血症这是一种败血症.

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