Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Sleep Apnea01:21

Sleep Apnea

Sleep apnea is a condition where breathing stops intermittently during sleep, often leading to significant health issues. Each episode can last from 10 to 20 seconds or more and is frequently accompanied by a brief arousal from sleep. This disturbance, largely unnoticed by the individual, can lead to severe daytime fatigue. Commonly, individuals seek help after being informed by their partners about loud snoring and noticeable breathing pauses during sleep.
The condition is more prevalent among...
Cardiopulmonary Resuscitation II: ACLS Airway Management01:22

Cardiopulmonary Resuscitation II: ACLS Airway Management

Airway management is a key skill in emergency and critical care settings, as maintaining a clear airway is essential for adequate oxygenation and ventilation.Head Tilt-Chin Lift TechniqueThe head tilt-chin lift maneuver is an essential technique primarily used in patients without suspected cervical spine injuries. To perform this maneuver, one hand is placed on the patient’s forehead, and gentle pressure is applied backward to tilt the head. The fingertips of the other hand are positioned under...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.

Analytical chemistry·2026
Same author

Barcoding for isobaric tagging of carboxylic acids and amines metabolites.

The Analyst·2026
Same author

Alkamines reveal a hidden layer of steroid and drug metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Spatial Mapping of the Precancer-to-Cancer Transition in Breast and Prostate.

Cancer discovery·2026
Same author

SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis.

Nature cell biology·2026
Same author

Metabolites from plasma-like medium fuel nitrogen metabolism and influence proliferation in <i>Leptospira interrogans</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
查看所有相关文章

相关实验视频

Updated: Jun 8, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K

使用96复数同位素标记的高通量代谢学.

Michael R Armbruster1, Scott F Grady1, Kevin Cho2

  • 1Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.

Analytical chemistry
|July 31, 2024
PubMed
概括
此摘要是机器生成的。

新的化学标签加快了液体染色学-质谱学 (LC-MS) 代谢,使得更快的分析和绝对量化. 这种方法有效地选哺乳动物细胞中的碳酸盐,克服了以前的工作流的限制.

更多相关视频

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

6.9K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

相关实验视频

Last Updated: Jun 8, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

6.9K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

科学领域:

  • 分析化学 分析化学
  • 代谢学 代谢学 代谢学
  • 生物化学 生物化学

背景情况:

  • 液体染色学-质谱学 (LC-MS) 是一种强大的代谢学工具.
  • 目前的LC-MS工作流程面临着长时间的分析时间和可变量化的挑战.
  • 矩阵效应和漫长的工作周期阻碍了高通量代谢学研究.

研究的目的:

  • 开发用于改善LC-MS代谢的新型化学标签.
  • 为了克服在代谢工作流程中延长工作周期的瓶.
  • 为了使代谢物的绝对量化,减少矩阵依赖.

主要方法:

  • 合成和应用96个独特的化学质量标签.
  • 使用LC-MS进行高通量代谢分析.
  • 在缺乏营养的哺乳动物细胞上进行代谢查.

主要成果:

  • 成功开发并实施了96个独特的化学质量标签.
  • 已证明可以缓解代谢工作流程瓶.
  • 实现了降低矩阵效应的代谢物绝对量化.
  • 在2小时内分析了288个样本,对碳酸的相对标准偏差 (RSD) 为24%.

结论:

  • 新型化学标签显著提高LC-MS代谢效率.
  • 这种方法可以快速准确地绝对量化代谢产物.
  • 该方法有效用于生物样本中的代谢查,例如哺乳动物细胞.