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绘制Shigella入侵等离子体抗原D (IpaD) 的功能B细胞表位

Siqi Li¹, Weiping Zhang¹

¹Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Applied and environmental microbiology

|July 31, 2024

概括

这项研究确定了来自Shigella细菌的入侵等离子体抗原D (IpaD) 的关键功能表位. 第1号和第5号情节显示,开发一种针对石病和痢疾的交叉保护性石疫苗具有前途.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
疫苗开发疫苗开发

背景情况:

希格拉菌在全球范围内引起严重的腹疾病,特别是在幼儿中.
第三类分泌系统 (T3SS) 对西格拉菌的入侵至关重要,入侵等离子体抗原D (IpaD) 是一个关键组成部分和潜在的疫苗点.
由于物种和血清型的异质性,目前对Shigella的疫苗开发是有限的,需要采用交叉保护方法.

研究的目的:

描述Shigella IpaD的功能性B细胞表位因其在细菌入侵和免疫性中的作用.
为了确定最佳的IpaD表位物,用于构建一种多价值疫苗来预防石病.
评估针对IpaD表位体产生的抗体在抑制Shigella入侵中的有效性.

主要方法:

在IpaD的连续B细胞表位的in silico预测.
预测表位体的融合到携带蛋白质,用于小鼠免疫.
在体外对抗IpaD IgG抗体反应及其对Shigella入侵的抑制活性的评估.

主要成果:

所有测试的IpaD表皮质融合蛋白都诱导了类似水平的抗IpaDIgG抗体.
对IpaD第1次表征 (SPGGNDGNSV) 和第5次表征 (SPNNTNGSSTET) 生成的抗体显示出Shigella flexneri 2a入侵的显著抑制.
第1和第5情节也显示出在防止Shigella sonnei.入侵方面提高了有效性.

结论:

IpaD表位1和5在诱导抑制Shigella入侵的抗体方面是有效的,使它们成为Shigella疫苗的合适候选人.
这些发现支持IpaD表位1和5在构建交叉保护性西格拉疫苗的多价蛋白抗原中的使用.
识别功能性表位体的方法可以广泛应用于开发针对其他异质病原体的疫苗.

关键词:

这就是IPAD.西格拉菌种类.标本地图绘制标本地图绘制入侵等离子体抗原的抗原.第三类分泌系统 (T3SS)疫苗疫苗疫苗疫苗

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