相关实验视频
Updated: Jun 18, 2025
11:09
Chromatin Isolation by RNA Purification ChIRP
Published on: March 25, 2012
86.2K
染色-seq在染色素标记处识别RNA
Ligang Fan1,2, Wei Sun, Yitong Lyu2
1Ministry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine, Northwest University, Xi'an, China.
Science advances
|July 31, 2024
概括
我们开发了Chrom-seq,一种新的方法来识别与活细胞中染色质标记相关的RNA. 这种技术有效地映射了参与表观遗传事件的调节性RNA,而不需要抗体.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 染色体标记调节基因转录,但识别相关的长非编码RNA (lncRNAs) 是具有挑战性的.
- 目前用于检测RNA-染色质关联的现有方法受到抗体要求和交叉连接的高噪声的限制.
研究的目的:
- 开发一种新的,高效的,无抗体的方法,用于捕获与活细胞中特定染色质标记直接相关的RNA.
- 系统地绘制涉及表观遗传调节的 lncRNA.
主要方法:
- 开发了Chrom-seq,将特定的染色体标记读取器与APEX2近距离标记相结合.
- 与染色质标记相邻的RNAs被生物化并使用斯特雷普塔维丁珠隔离.
- 使用了H3K27me3,H3K9me3和H3K4me3标记的读取器.
主要成果:
- Chrom-seq成功检测出与H3K27me3,H3K9me3和H3K4me3.3相关的RNA物种.
- 与现有技术相比,该方法显示出更高的灵敏度,效率和成本效益.
- 在特定的表观遗传标记上确定了潜在的调节性RNA.
结论:
- Chrom-seq提供了一种强大的,无抗体的方法,用于在活细胞中映射染色质关联RNA.
- 这种方法促进了在表观遗传调节中对lncRNAs的系统研究.
- 能够更深入地了解RNA在染色体组织和功能中的作用.
相关概念视频
02:36Chromatin Immunoprecipitation- ChIP
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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03:21RNA-seq
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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