在小鼠卵细胞成熟过程中,甲暴露会损害G2/M和甲相-亚相过渡
Zhen-Nan Pan1, Li-Li Zhuang1, Hui-Shan Zhao1
1Reproductive Medicine Center, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, China; Shandong Provincial Key Medical and Health Laboratory of Reproductive Health and Genetics (Yantai Yuhuangding Hospital), China.
Ecotoxicology and environmental safety
|July 31, 2024
概括
甲 (PrPB) 是一种内分泌干扰剂,通过引起介质性停止和极体挤出失败,损害了小鼠卵细胞的成熟. 这种防腐剂破坏了线粒体功能,DNA完整性和组装,影响生殖健康.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 甲 (PrPB) 是一种广泛使用的防腐剂,具有已知的内分泌干扰特性.
- 在人类样本中检测到PrPB,并与生殖功能下降有关.
- PrPB对哺乳动物卵细胞成熟的特定影响在很大程度上仍未被描述.
研究的目的:
- 在实验室中研究甲 (PrPB) 对小鼠卵细胞成熟的直接影响.
- 阐明 PrPB 诱导的卵细胞发育缺陷背后的分子机制.
主要方法:
- 在实验室中,小鼠卵细胞暴露在PrPB的不同度中.
- 介质恢复,生殖囊泡分解 (GVBD) 和极体挤出的评估.
- 分析线粒体功能,氧化应激,DNA损伤和/染色体组织.
主要成果:
- 暴露于PrPB (600μM) 显著降低了GVBD的发病率,并导致中介性恢复停止.
- PrPB诱导了线粒体功能障碍,氧化应激和卵细胞中的DNA损伤.
- PrPB破坏了轴心形态学,染色体对齐和动态管-微管附着,激活了轴心组装检查点 (SAC).
结论:
- PrPB通过破坏关键细胞周期过渡,对小鼠卵细胞成熟产生不利影响.
- 机制包括线粒体功能受损,DNA损伤和异常的螺旋染色体动力学.
- 这些发现凸显了PrPB对女性生殖健康的潜在威胁.
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