带有甲酸和甲基棕酸的脂质体:开发,表征,稳定性评估,体外安全性概况,抗菌和抗氧化活性
Fernanda Reis Favarin1, Éricles Machado Forrati1, Vitória Almeida Bassoto1
1Laboratory of Nanotechnology, Universidade Franciscana, Santa Maria, Rio Grande do Sul, Brazil.
Food chemistry
|July 31, 2024
概括
这项研究开发了与 Askorbic 酸和 Askorbyl Palmitate 共同封装的稳定脂质体,显示出强大的抗氧化活性和无毒性. 这些脂质体在冷藏下保持其性能60天.
科学领域:
- 制药科学 制药科学
- 材料科学 材料科学 材料科学
背景情况:
- 亚酸 (AA) 和亚甲基棕酸 (AP) 是具有治疗潜力的重要抗氧化剂.
- 脂质体封装可以提高活性化合物的稳定性和输送.
- 联合交付系统需要精心制定,以获得最佳的疗效和稳定性.
研究的目的:
- 准备和表征脂体共封装亚斯科伯酸 (AA) 和亚斯科伯酸棕酸 (AP).
- 评估已开发的脂质体的稳定性,细胞毒性,抗氧化剂和抗菌活性.
- 为了优化脂质体配方,提高稳定性和抗氧化性质.
主要方法:
- 进行了前配方研究,以优化脂质体组成和特征.
- 脂质体配方LIP-AAP的特征是囊泡大小,多分散度指数,泽塔潜力和pH.
- 确定了封装效率和AA和AP的含量.
- 评估了冷藏下的稳定性,细胞毒性,抗氧化活性和抗菌作用.
主要成果:
- 优化的LIP-AAP配方表现出囊泡大小为161nm,多分散度指数为0.215,泽塔电位为-31.7mV,pH为3.34.
- 封装效率为AA的37%,AP的79%,每个含量为1mg/ml.
- 脂质体在冷藏下稳定60天,具有显著的抗氧化活性 (31.4μMol TE/mL),并且没有细胞毒性.
- 对测试的细菌和真菌没有观察到抗菌作用.
结论:
- 同封装脂质体 (LIP-AAP) 是一种强大而稳定的抗氧化剂.
- 优化的配方在最佳储存条件下保持了物理和化学性能.
- 脂质体显示出作为有效的抗氧化剂输送系统的承诺,尽管在本研究中没有抗菌作用.
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