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使用脉冲电磁波运动生物反应器系统对人类介质干细胞进行转录学分析,以增强骨质生成承诺和治疗潜力

Aayushi Randhawa¹, Keya Ganguly², Sayan Deb Dutta³

¹Department of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea; Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, 24341, Republic of Korea.

|July 31, 2024

概括

这项研究引入了一种使用脉冲电磁场 (pEMF) 和波动的新型生物反应器,以增强人类骨髓衍生中介质干细胞 (hBMSC) 培养,促进治疗应用的增殖和骨质生成潜力.

科学领域:

生物技术是生物技术.
再生医学是一种再生医学.
细胞培养工程细胞培养工程

背景情况:

使用3D支架或聚合物的传统生物反应器限制了细胞分泌的生物分子的产生.
扩展性和高效的培养间酶体干细胞的方法对于治疗应用至关重要.

研究的目的:

研究一种新型脉冲电磁场 (pEMFs) 辅助波动生物反应器的疗效,用于培养人类骨髓衍生细胞干细胞 (hBMSC).
评估动态pEMF (D-pEMF) 对hBMSC增殖,骨质生分化和治疗因素分泌的影响.

主要方法:

在波动生物反应器中培养hBMSCs与D-pEMF刺激 (10V-1Hz,5.82 G,35 OPM30分钟).
评估细胞增殖动力学和骨质基因表达.
利用RNA测序来分析基因表达在对D-pEMF的反应中发生的变化.

主要成果:

与静态培养相比,D-pEMF显著增强了hBMSC的增殖 (1x10^5到4.5x10^5).
D-pEMF培养表明骨质转录因子和基因表达的增加,表明骨质生成的增强.
D-pEMF刺激还诱导了hBMSCs的ROS清理特性.

结论:

波动和D-pEMF生物反应器系统的结合促进了hBMSC的快速扩散,并诱导了骨质性质.
这种方法为基于细胞的疗法提供了一种生物活性,具有成本效益和潜在的更安全的方法,不需要昂贵的材料.

关键词:

以及骨质生成.生物物理刺激是生物物理刺激.介质细胞干细胞介质细胞干细胞脉冲电磁场是脉冲的电磁场.波动生物反应器是一种波动生物反应器.

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...