整体基因组序列分析Reticuloendotheliosis病毒集成在非同类的avipoxvirus中
Huadong Liu1, Tingting Li1, Juan Tang1
1College of Animal Science, Shanxi Agricultural University, Taiyuan, 030032, China.
Microbial pathogenesis
|July 31, 2024
概括
来自山西省太原市的所有水和水病毒菌株都显示出完全集成的网状尾症病毒 (REV) 序列. 这些集成的REV序列具有很高的遗传相似性,这表明鸟类天花病毒的共同起源或传播途径.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 鸟类病理学 鸟类病理学
背景情况:
- 鸟病毒 (APV) 通常集成Reticuloendotheliosis病毒 (REV) 核酸序列.
- 了解麻疹病毒 (FPV) 和白病毒 (PPV) 的遗传特征对于疾病控制至关重要.
研究的目的:
- 在山西省太原分离的FPV和PPV菌株的特征.
- 为了确定基因组相似性,并在这些鸟类天花病毒分离物中确定集成的REV序列.
主要方法:
- 在胚胎中隔离和培养疑似FPV和PPV菌株.
- 聚合酶链反应 (PCR) 放大4b核心蛋白质基因和ORF201和ORF203.3之间的序列.
- 用DNA测序和比较序列分析来确定基因组的同一性和相似性.
- 完整的集成REV序列的放大和序列化.
主要成果:
- 确定了四种FPV菌株 (A1型) 和一种PPV菌株 (A2型),它们彼此之间以及与其他中国分离物具有高度相同性.
- FPV菌株与FWPV-09-Jilin百分之百相同,与B2型病毒具有较低的相同性 (74%).
- PPV菌株与本地FPV菌株相同90.1%,与其他A2型PPV菌株相同100%.
- 完整的REV序列 (7942-8005bp) 被整合到所有隔离的FPV和PPV中,显示出与来自中国各地区的REV隔离物高度相同.
- 综合的REV序列属于III型,与株APC-566和CY1111具有很高的相同性 (99.9%),并且在多基因中存在显著的变异.
结论:
- 在山西省太原分离的所有FPV菌株都具有完全集成的REV序列,具有高度的菌株内同一性.
- 该PPV隔离物还包含一个完全集成的REV序列,与集成到本地FPV菌株中的REV具有高度相同性.
- 这些发现突显了该地区鸟类天花病毒中集成REV序列的流行率和遗传保护.
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