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一个三维的芯片上的门微生理系统涉及细胞循环进展,胆固醇代谢和蛋白质平衡在早期的状动脉病进展

Ishita Tandon¹, Alan E Woessner², Laίs A Ferreira¹

¹Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, USA.

Acta biomaterialia

|July 31, 2024

概括

研究人员使用双层水凝开发了一种3D芯片上的门模型,用于研究状动脉病 (CAVD). 这种模型通过模仿大动脉膜来揭示早期疾病机制.

科学领域:

生物材料科学生物材料科学
心血管研究研究心血管研究
组织工程是组织工程.

背景情况:

动脉疾病 (CAVD) 是一种常见的膜病变,致死性心血管事件的风险增加了50%.
目前的治疗依赖于门置换,因为早期诊断和治疗策略尚未开发.
有效的体外模型对于了解早期CAVD机制和开发新干预措施至关重要.

研究的目的:

开发一个生理上相关的3D芯 (VOC) 系统来建模CAVD.
使用生物仿真体外系统研究早期CAVD机制.
为评估潜在的诊断和治疗策略提供一个平台.

主要方法:

制造一个多层,双层的水凝系统,模仿大动脉外细胞矩阵 (ECM) 组成.
将猪大动脉间歇细胞 (VIC) 和内皮细胞 (VEC) 纳入共同培养.
动态机械刺激的应用和使用多光子成像和蛋白质组分析进行评估.

主要成果:

基于原的双层水凝成功维持了VIC表型.
蛋白质组分析揭示了与细胞循环,胆固醇生物合成和蛋白质平衡相关的蛋白质的显著变化,这些变化发生在患病的VOC中.
这些变化与细胞代谢的变化相关,表明早期的适应性疾病发病阶段.

结论:

开发的VOC系统有效地模仿了健康和生病的大动脉组合.
患病的VOC为CAVD的启动过程提供了关键的见解.
这些发现支持VOC作为研究早期CAVD和开发新型治疗方法的宝贵工具.

关键词:

原-GAG水凝是一种原-GAG水凝.微生理系统微生理系统多光子显微镜的使用方法器官在芯片上的器官蛋白质表达方式蛋白质表达方式门细胞的新陈代谢

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