结肠直肠癌和炎症性肠病具有共同的唾液蛋白质路径
Nicole Contran1, Giorgio Arrigoni2, Ilaria Battisti2
1Department of Medicine (DIMED), University of Padova, 35128, Padova, Italy. nicole.contran@studenti.unipd.it.
唾液和便的生物标志物可以检测炎症性肠病 (IBD) 和结直肠癌 (CRC). 在克罗恩氏病 (CD) 便中发现的某些可以促进CRC细胞的生长,这表明它们在疾病发展中的作用.
科学领域:
- 生物化学 生物化学
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
背景情况:
- 炎症性肠病 (IBD) 显著增加结直肠癌 (CRC) 的风险.
- 早期IBD和CRC检测的非侵入性生物标志物至关重要.
研究的目的:
- 在唾液和便中识别IBD和CRC的非侵入性蛋白质和类生物标志物.
- 研究特定的唾液在CRC细胞增殖中的作用.
主要方法:
- 在对照组,IBD和CRC患者的唾液和便样本上的非向蛋白质组 (LTQ-Orbitrap/MS).
- 生物信息分析 (g-Profiler) 用于蛋白质功能和路径丰富.
- 在体外测试评估特定对CRC细胞系和信号通路的影响.
主要成果:
- 鉴定了152种唾液蛋白;73种是IBD和CRC共享的,与细胞粘附和酶调节有关.
- 在克罗恩病 (CD) 中高表达的14种蛋白质和CRC中的11种蛋白质,与DNA复制和免疫有关.
- CD便含有唾液 (PRBs),通过Erk1/2,Akt和p38通路刺激CRC细胞增殖.
结论:
- 在IBD和CRC之间存在用于DNA稳定性和免疫力的共享唾液蛋白质签名.
- 在CD便中的唾液PRB衍生可以通过促进通过瘤性途径的细胞增殖促进CRC病原发生.
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