Paenibacillus gyeongsangnamensis sp. 这种菌种的种类是什么? nov., 从土壤中分离出来
Hyosun Lee1, Dhiraj Kumar Chaudhary2, Dong-Uk Kim1
1Department of Biological Science, College of Science and Engineering, Sangji University, Wonju 26339, Republic of Korea.
Journal of microbiology and biotechnology
|August 1, 2024
概括
从土壤中发现了一种新型细菌物种Paenibacillus gyeongsangnamensis. 这种有氧的,格拉姆阳性细菌扩大了Paenibacillus属内的已知多样性.
科学领域:
- 微生物学 微生物学
- 细菌的分类学 细菌的分类学
- 基因组学就是基因组学.
背景情况:
- 帕尼巴西勒斯 (Paenibacillus) 属包括各种各样的细菌物种,具有不同的生态作用.
- 对新型细菌分离物的准确分类对于理解微生物多样性和功能至关重要.
研究的目的:
- 从土壤中描述和分类一种新分离的细菌菌株,被指定为dW9T.
- 确定dW9T菌株在Paenibacillus属中的分类地位.
主要方法:
- 从土壤中分离和培养dW9T菌株.
- 生理和生化表征 (格拉姆染色,催化酶,氧化酶测试,生长条件).
- 使用16S rRNA基因测序进行的家族遗传学分析.
- 基因组分析包括基因组大小,DNA G+C含量,数字DNA-DNA杂交 (dDDH) 和平均核酸标识 (ANI).
- 呼吸系统和主要脂肪酸的分析.
主要成果:
- 菌株dW9T是一种格拉姆阳性,有氧,白色,杆状的细菌.
- 遗传学分析将dW9T菌株置于Paenibacillus属内,其中Paenibacillus filicis S4T是最接近的亲属 (97.4%的16S rRNA相似性).
- 基因组数据 (dDDH <22.0%,ANI <74.0%) 和化学分类学特征 (MK-7,antiso-C15:0,iso-C16:0) 支持其作为新物种的区别.
- 基因组大小为7,787,916bp,DNA G+C含量为51.3%.
结论:
- 菌株dW9T符合将其归类为Paenibacillus属中的新物种的标准.
- 这种新物种的拟议名称是Paenibacillus gyeongsangnamensis sp. 在新的一年里.
- 类型菌株是dW9T (=KCTC 43431T) =NBRC 116022T) 的.
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