转录因子EjNAC5调节了水果冷却的化
Yiqing Huang1, Zihao Liang1, Jiao Lu1
1College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Journal of experimental botany
|August 1, 2024
概括
冷却压力会导致洛奎特水果的木质化,影响质量. 这项研究确定了EjNAC5作为一个关键的调节器,其促进物甲基化控制了冷藏期间的素水平和水果坚硬度.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 冷却压力会影响植物生理学,往往导致不良变化,如水果的木质化.
- 在冷藏期间,红肉水果表现出更高的坚硬度和素含量,这会对食用质量产生负面影响.
- 基因甲基化和素生物合成与植物对环境压力的反应有关.
研究的目的:
- 为了研究红肉水果冷却诱导的化背后的分子机制.
- 确定关键的基因和调节途径,参与控制水果坚硬性和冷藏期间的素积累.
- 探索DNA甲基化在调节冷却诱导的化中的作用.
主要方法:
- 综合RNA测序和全基因组双硫酸盐测序分析在不同储存温度 (0°C与5°C) 下对洛卡特水果进行测序.
- 鉴定和表征EjNAC5基因及其促进子区域.
- 基因表达分析,在鱼,阿拉比多普西斯和肝草中进行过度表达的暂时和稳定的实验,以及蛋白质-蛋白质相互作用测试.
主要成果:
- 5°C的储存有效地减轻了在0°C时在"Dahongpao"水果中观察到的度和素含量的增加.
- EjNAC5转录水平与水果坚硬度正相关,与促进体DNA甲基化水平负相关.
- 过度表达EjNAC5增加了素含量;EjNAC5与EjERF39和EjHB1相互作用,激活了素生物合成基因 (Ej4CL1,EjPRX12).
结论:
- EjNAC5是一种关键的转录因子,它调节了洛卡特水果的冷却诱导的化.
- 通过EjNAC5促进体甲基化进行表观遗传调节,调节其转录水平和随后的素生物合成.
- 新的调节模块 (EjNAC5-EjERF39-Ej4CL1和EjNAC5-EjHB1-EjPRX12) 在冷却诱导的化途径中被确定.
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