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Updated: Jun 18, 2025

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

在宫癌研究中的组织微阵列验证. 一种方法论性的方法.

Lucília Lovane^1,2,3, Carla Carrilho^2,3, Christina Karlsson⁴

¹School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden. Lucilia.Matias@oru.se.

Histology and histopathology

|August 1, 2024

概括

此摘要是机器生成的。

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组织微阵列 (TMA) 可以准确地代表整个幻灯片图像 (WSs) 用于宫癌生物标志物研究. 三个0.6毫米芯足以进行可靠的分析,验证TMA的成本效益.

科学领域:

在瘤学瘤学.
病理学病理学病理学
生物标志物研究生物标志物研究

背景情况:

子宫癌 (CC) 是一种普遍存在的女性癌症,特别是在资源较低的环境中,通常与人乳头瘤病毒有关.
生物标志物对于CC诊断,治疗和预后至关重要.
组织微阵列 (TMA) 为临床研究中的生物标志物研究提供了一种具有成本效益的方法.

研究的目的:

评估和验证TMA在子宫癌研究中的有效性,与整个幻灯片图像 (WSs) 相比.
为了确定TMA核心的最佳数量,以准确地表示生物标志物.
建立在各种研究环境中对TMA采样的通用方法.

主要方法:

选择和匿名的20例子宫癌病例.
通过免疫组织化学研究p16,细胞素5 (CK5),细胞素7 (CK7),编程死亡配体1 (PD-L1) 和CD8的表达.
扫描整个幻灯片图像 (WSs) 并创建虚拟的TMA核心 (直径0.6毫米).
对每个生物标志物进行了评估,对每例1-5个气的十个随机组合进行了评估.
使用kappa统计数据评估了TMA和WS之间的评分协议.

主要成果:

三个0.6毫米的TMA核心准确地代表了这个宫癌队列中的WS发现.

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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

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Last Updated: Jun 18, 2025

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

对于TMA和WS之间的协议,卡帕价值在1 (p16) 到0.61 (PD-L1) 之间.

证明了TMA在评估宫癌多个生物标志物的可靠性.

结论:

组织微阵列 (TMA) 是用于宫癌生物标志物研究的经过验证和有效的工具,为整个幻灯片图像提供了具有成本效益的替代方案.
使用三个0.6毫米核心的采样策略可提供可靠的生物标志物表达的表现.
提议的TMA采样方法可以对各种组织和生物标志物进行概括,从而提高临床研究的效率.