在科威特医院中对甲素敏感的金黄色葡萄球菌 (MSSA) 隔离物的基因组分析
Samar S Boswihi1, Wadha A Alfouzan1,2, Edet E Udo1
1Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait.
Frontiers in microbiology
|August 1, 2024
概括
这项研究调查了科威特医院中对甲素敏感的金黄色葡萄球菌 (MSSA),揭示了由特定克隆主导的多样化的遗传背景. 这些发现突显了这些MSSA克隆的长期稳定性,为感染控制策略提供了关键数据.
科学领域:
- 临床微生物学和传染病
- 细菌病原体的分子流行病学
背景情况:
- 黄金葡萄球菌是社区和医院获得感染的重要原因.
- 关于科威特医院中甲素敏感黄金葡萄球菌 (MSSA) 的遗传多样性和抗生素耐药性概况的数据有限.
- 了解MSSA的克隆组成对于有效的感染预防和控制至关重要.
研究的目的:
- 调查来自科威特医院的MSSA分离物的抗生素耐药性模式和遗传背景.
- 确定MSSA殖民或感染患者的克隆分布和组成.
- 为未来的监测,建立科威特MSSA流行病学的基线数据.
主要方法:
- 在2016年和2021年期间,从13家科威特公立医院的患者中收集连续的MSSA隔离物.
- 使用抗生素图谱分析,葡萄球菌蛋白A (spa) 类型,DNA微阵列分析和多位元序列类型 (MLST) 的表征.
- 使用标准化实验室协议对446个MSSA分离物的分析.
主要成果:
- 所有的MSSA分离物仍然对万科米辛,提科普拉宁,线化物,塞法托林,里法素和穆皮罗辛敏感.
- 对红红素 (21.3%),克林达胺素 (14.0%),酸 (27.0%),三甲 (23.1%),和西普罗夫洛素 (35.2%) 观察到显著的耐药性.
- 分子类型确定了155个spa类型,31个克隆复合体 (CC) 和56个序列类型 (ST),其中CC1,CC15,CC30和CC361占主导地位.
结论:
- 科威特医院的MSSA分离体表现出多样化的遗传背景,主要由CC1,CC15,CC30和CC361驱动.
- 克隆分布在2016年至2021年期间保持相对稳定,这表明持续存在的特有克隆.
- 这项研究提供了第一份关于科威特MSSA基因组合的综合数据,这对于有针对性的干预至关重要.
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