阴极pH和生物增加对微生物电合成细胞中酸盐和CH4的产生的影响
Emmanuel Nwanebu1, Mara Jezernik2, Christopher Lawson2
1Energy, Mining and Environment Research Centre, National Research Council Canada 6100 Royalmount Avenue Montreal Quebec H4P 2R2 Canada Boris.Tartakovsky@cnrc-nrc.gc.ca.
RSC advances
|August 1, 2024
概括
低pH值增强微生物电合成 (MES) 细胞中的酸盐生产. 用乙酸性细菌进行生物增量改善了乙酸盐产量,但系统内的选择性压力是增加产量的主要驱动因素.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 电化学 电化学 电化学
背景情况:
- 微生物电合成 (MES) 为二氧化碳转化提供了一条途径.
- 优化pH值和微生物种群对于MES中高效的产品形成至关重要.
- 乙酸盐生产是微生物转化二氧化碳的关键目标.
研究的目的:
- 在不同pH值 (5.3, 7, 8) 的MES细胞中比较二氧化碳转化效率.
- 评估生物增量与特定的乙酸性细菌对乙酸盐生产的影响.
- 研究选择性压力与直接生物增强在增强微生物转化中的作用.
主要方法:
- 运行两个100毫升的MES电池,连续供应碳酸盐.
- 来自无氧化消化器污泥的野生类型或生物增强混合微生物种群的注射细胞.
- 分析了酸盐和甲生产率,并进行了16S rRNA基因测序.
主要成果:
- 在低pH值 (5.3) 时,乙酸盐的产量最高,达到1.0g (Lcd) -1,尽管甲的产量仍然存在.
- 生物增量使乙酸的产量增加到2.0g (Lcd) -1,但其他碳酸的含量仍然很低.
- 16S rRNA测序表明引入的细菌密度较低,这表明选择性压力驱动了乙酸盐增强.
结论:
- 低pH值是碳酸盐养MES中酸盐生产的最佳条件.
- 虽然生物增量显示出潜力,但MES系统内的选择性压力对改善乙酸产量更为关键.
- 进一步的研究可能将重点放在优化条件上,以提高引入微生物菌株的性能.
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