描述和应用Bacillus velezensis D6共同产生α-氨酶和蛋白酶的特性
Zong-Min Wang1, Shuang Wang1, Hua Bai1
1School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China.
Journal of the science of food and agriculture
|August 1, 2024
概括
百菌 velezensis D6有效地共同产生α-氨酶和蛋白酶,表现出优异的热稳定性和广泛的pH活性. 这些酶对粉和蛋白质基质都有显著的水解能力,突出显示了它们的工业潜力.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 微生物发酵 微生物发酵
背景情况:
- 有限的研究存在于Bacillus velezensis多种酶的共同生产.
- 了解像B. velezensis这样的新物种中的多酶生产对于生物技术应用至关重要.
研究的目的:
- 调查B. velezensis D6的发酵特性,以共同产生α-氨酶和蛋白酶.
- 探索这些共同产生的酶在发酵中的酶性质和应用潜力.
主要方法:
- 优化了B. velezensis D6.6的发酵条件
- 分析了酶活性,基因共同表达,热稳定性和pH稳定性.
- 对玉米,大米,,大豆和鱼等各种基质的水解效应进行了评估.
主要成果:
- 在优化条件下实现了最大的α-氨酶 (13.13 U/mL) 和蛋白酶 (2106.63 U/mL) 联合生产.
- 共产的酶表现出极好的热稳定性 (在90°C,1小时后保持超过80%的活性) 和广泛的pH稳定性.
- 通过α-amylase证明了粉性材料的显著水解和动物蛋白质的蛋白质酶的优先水解.
结论:
- B. velezensis D6 是阿尔法-氨酶和蛋白酶的联合生产的一个有希望的候选者.
- 这项研究系统地阐明了这些酶的共同生产和特性,扩大了对B. velezensis能力的了解.
- 这些发现支持这些酶在发酵过程中的潜在工业应用.
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