最近在基于移动波的高分辨率离子移动性分离方面的进展与质谱学相结合
1Department of Chemistry, University of Utah, Salt Lake City, Utah, USA.
Mass spectrometry reviews
|August 1, 2024
概括
高分辨率离子移动光谱-质谱 (IMS-MS) 的进步,包括循环 (cIMS) 和无损离子操纵结构 (SLIM),为奥米克工作流提供了增强的分离. 这些技术改善了对甘氨酸,脂质和代谢物的分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 离子移动性光谱-质谱 (IMS-MS) 越来越多地集成到omics工作流程中.
- 最近的仪器仪表进步显著改善了离子移动性分离分辨率.
- 循环IMS (cIMS) 和无损离子操纵结构 (SLIM) 是关键的TWIMS平台,可以实现高分辨率的分离.
研究的目的:
- 审查基于高分辨率移动波的IMS-MS的最新发展 (2019-2023年).
- 为了突出仪器仪表,校准,字符串技术和应用方面的进步.
- 展示高分辨率IMS-MS在分析复杂的生物样本中的实用性.
主要方法:
- 专注于cIMS和SLIM IMS-MS的仪器仪表开发,利用延长的路径长度.
- 讨论校准策略,以提高准确性和可靠性.
- 通过染色学,成像学和冷红外光谱学等技术探索连线.
主要成果:
- 展示了可以使用扩展的拼接长度TWIMS实现的高分辨率分离.
- 在多通道分离中计算碰撞截面 (CCS) 值的成功应用.
- 有效的异构分离甘氨酸,脂质和小代谢物.
结论:
- 基于高分辨率移动波的IMS-MS平台 (cIMS,SLIM) 是奥米克研究的强大工具.
- 仪器仪表和连线技术的进步扩大了IMS-MS的分析能力.
- 这些技术为复杂的生物分子的分离和表征提供了关键的见解.
关键词:
离子流动性光谱仪离子流动性光谱仪这些同位素是同位素.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量分离科学科学 分离科学更多相关视频
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
597
16:40T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
Published on: July 31, 2010
24.6K
相关概念视频
Mass Spectrometry: Complex Analysis
737
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
737
High-Resolution Mass Spectrometry (HRMS)
1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
Mass Analyzers: Common Types
585
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
585
Mass Analyzers: Overview
625
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
625
Electrospray Ionization (ESI) Mass Spectrometry
768
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
768
Tandem Mass Spectrometry
943
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
943
