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微封装重组LAB载体表达光报告蛋白的有针对性的生物成像:微生物追踪的非侵入性方法
Prakash Biswas1, Afruja Khan1, Amirul Islam Mallick1
1Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, West Bengal 741246, India.
ACS biomaterials science & engineering
|August 1, 2024
概括
研究人员设计了Lactococcus lactis细菌来表达光蛋白质,并将它们封装在酸盐中. 这种方法允许在体内扩展,非侵入性跟踪活肠道微生物.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 胃肠病学 胃肠病学
背景情况:
- 乳球菌 (L. lactis) 是一种GRAS细菌,以其健康益处和蛋白质表达而闻名.
- 在体内跟踪微生物生物分布对于理解益生菌效应至关重要,但具有挑战性.
- 目前的方法,如化学结合,是昂贵的和耗时的.
研究的目的:
- 开发一种实时,体内追踪非共生益生菌细菌的方法.
- 在复杂的肠道环境中研究转基因L. lactis的生物分布和命运.
- 为了提高L. lactis in vivo的稳定性和检测性.
主要方法:
- 经过基因工程改造的L. lactis能够表达mCherry和mEGFP光记者蛋白.
- 微封装的工程L. lactis使用奇托与三聚酸盐 (TPP) 交叉连接.
- 在体外和体内 (鸟类和小鼠模型) 评估了记者蛋白表达,光检测和胃内稳定性.
主要成果:
- 奇托-TPP涂层在重组L. lactis中保持了稳定的记者蛋白表达 (rL. 牛奶). 牛奶). 牛奶. 这就是牛奶.
- 光检测特异性和灵敏度不受涂层的影响.
- 素-TPP涂层增强了胃内稳定性,使得扩展的时空分布研究成为可能.
结论:
- 基因改造与素封装相结合,提供了一种稳定有效的方法来追踪非共生LAB.
- 这种方法为肠道中活微生物的追踪提供了一个有希望的非侵入性工具.
- 开发的技术有助于在体内深入研究微生物动态.
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