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用于合成L-酸盐的多酶系统的构建和应用

Jielin Zhao^1,2,3, Xiaolian Li^1,3, Ranfeng He^1,3

¹State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

Applied biochemistry and biotechnology

|August 1, 2024

概括

这项研究开发了一种多酶系统,用于高效的L-酸盐合成,使用工程化大肠杆菌. 优化的全细胞生物催化剂实现了高L-酸盐度和从酸盐的转化率.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
代谢工程是代谢工程.

背景情况:

酸是一种有价值的有机酸,具有多种工业应用.
目前的L-酸盐生产方法在效率和成本效益方面面临挑战.
开发强大的酶系统对于可持续的L-酸盐合成至关重要.

研究的目的:

设计一种用于生产L-酸盐的多酶系统.
为了优化酶表达和共同表达在大肠杆菌.
建立一个高效的全细胞生物催化工艺,用于L-酸盐合成.

主要方法:

重组大肠杆菌菌株的构建,表达雄性酸cis-trans异构酶 (MaiA) 和烟酶C (FumC).
优化协同表达系统并选择具有最高活性菌株 (pFM2).
料批发发酵用于增强酶活性和开发全细胞催化过程.

主要成果:

塞拉蒂亚马尔塞森 MaiA (SMaiA) 和大肠杆菌FumC (ECFumC) 显示出高的催化性能.
工程化大肠杆菌菌株pFM2在食批发发酵后显著增强了MaiA和FumC活动.
使用菌株pFM2的全细胞工艺在4小时内将1mol/L的酸盐转化为143.8g/L的L-酸盐,其摩尔转化率为98.4%.

结论:

成功开发了一种高效的L-酸盐合成多酶系统.
工程化大肠杆菌菌株pFM2作为一个强大的全细胞生物催化剂,用于工业L-酸盐生产.
这项研究为酶性L-酸盐合成的大规模工业应用提供了基础.

关键词:

l-马拉酸盐是一种共同表达是一种共同表达.吸烟剂的使用方法酸马利酸转化异构酶多酶催化剂的催化作用

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