在人血清样本中量化胆固醇相关的固醇和建立决策支持系统的整合性协议
Eva Kočar1, Sonja Katz2, Žiga Pušnik3
1Centre for Functional Genomics and Bio-Chips, Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Zaloška cesta 4, 1000 Ljubljana, Slovenia.
STAR protocols
|August 1, 2024
概括
本研究详细介绍了一种测量血清中胆固醇固醇的方法,使用液体染色学-并联质谱法 (LC-MS/MS). 还提出了机器学习模型,通过代谢途径分析来改善疾病监测.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 计算生物学 计算生物学
背景情况:
- 临床诊断越来越多地使用代谢生物标志物.
- 与胆固醇相关的固醇是代谢途径的关键指标.
- 精确量化固醇对于疾病监测至关重要.
研究的目的:
- 介绍人类血清中固醇分析的综合方案.
- 描述机器学习在疾病监测中的新型决策系统中的应用.
- 为了能够使用目标LC-MS/MS.精确测量10个固醇.
主要方法:
- 样品制备和醇提取的协议.
- 通过向液体染色学-并联质谱法 (LC-MS/MS) 量化10种与胆固醇相关的固醇.
- 实现用于预测建模的机器学习技术.
主要成果:
- 一个验证的醇提取和量化的协议.
- 证实LC-MS/MS的潜力,用于精确测量固醇.
- 开发用于疾病监测应用的机器学习模型.
结论:
- 提出的协议有助于在血清中进行可靠的类固醇分析.
- 机器学习集成为代谢途径监测提供了先进的工具.
- 这种方法支持加强临床诊断和疾病管理.
相关概念视频
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
143
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
143
01:29Cholesterol: Significance and Regulation
524
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
524