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毒素检测和耐药性的融合进化为保存的细菌-真菌相互作用提供了证据

Stephen K Dolan^1,2,3,4, Ashley T Duong^1,4, Marvin Whiteley^1,3,4

¹School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30310.

Proceedings of the National Academy of Sciences of the United States of America

|August 1, 2024

概括

Pseudomonas aeruginosa 检测并防御由Aspergillus fumigatus产生的有毒质毒素. 这种防御涉及一种独特的滴醇氧化酶 (DnoP),可以排毒毒素,展示微生物防御策略的融合进化.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

微生物形成复杂的社区,需要复杂的生存策略.
Pseudomonas aeruginosa 经常与像 Aspergillus fumigatus 这样的真菌共同感染.
了解微生物相互作用是解读社区功能的关键.

研究的目的:

识别和描述 Pseudomonas aeruginosa 防御质毒素的分子机制.
研究在这种防御反应中的平衡作用.
探索跨王国毒素防御机制的进化趋同.

主要方法:

对 Pseudomonas aeruginosa 的转录分析,作为对 Gliotoxin 暴露的反应.
滴醇氧化酶DnoP的鉴定和表征.
酶分析测定DnoP的排毒活性.
对DnoP和Aspergillus fumigatus抗毒素蛋白 (GliT) 的比较分析.

主要成果:

Gliotoxin 破坏了 Pseudomonas aeruginosa 的稳定,从而触发了新型二醇氧化酶 DnoP 的转录激活.
DnoP有效地排毒质毒素和相关的二硫化物含有毒素.
DnoP的酶机制在功能上与来自Aspergillus fumigatus的GliT相同,尽管其低序列同质性.
低水平的DnoP诱导表明了一个保存的环境传感机制.

结论:

Pseudomonas aeruginosa 具有针对 Aspergillus fumigatus 衍生的质毒素的特定防御机制.
DnoP代表了毒素耐药性和环境暗示传感在真菌和细菌王国之间融合进化的罕见实例.
这些发现突出了使多微生物在自然环境中生存的复杂分子适应.

关键词:

亚斯伯吉拉斯烟虫 (Aspergillus fumigatus) 是一种有毒的植物.伪omonas aeruginosa 这种类型的病毒.囊性纤维化症是什么类毒素 (gliotoxin) 是一种可怕的毒素.多微生物的相互作用.

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