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一种新的II型CHH神经涉到薄荷的卵巢发育,Lysmata vittata vittata

Huiling Lan¹, Fang Liu¹, Li Lu¹

¹State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen, China.

|August 1, 2024

概括

甲类高血糖激素 (CHH) 神经Lvit-GIH2抑制了的卵巢发育. 这种激素抑制关键基因表达,并负面调节生殖生长必不可少的途径.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
甲动物的繁殖甲动物的繁殖

背景情况:

第二种类型的甲类高糖激素 (CHH) 神经调节甲类动物的各种生理过程.
两种II型CHH转录,MIH/GIH1 (Lvit-GIH1) 和MIH/GIH2 (Lvit-GIH2),在雌雄性 Lysmata vittata中被确定.
已知Lvit-GIH1可以抑制卵巢发育.

研究的目的:

为了研究Lvit-GIH2在Lysmata vittata的卵巢发育中的作用.
描述Lvit-GIH2基因及其表达模式.
阐明Lvit-GIH2影响卵巢功能的分子机制.

主要方法:

对Lvit-GIH的转录组测序和开放式读取框架 (ORF) 分析2.
定量实时PCR (qRT-PCR) 分析不同性阶段的Lvit-GIH2表达.
RNA干扰 (RNAi) 和重组蛋白 (rGIH2) 实验,以评估对基因表达的影响 (Lvit-Vg,Lvit-VgR).
在rGIH2治疗后对卵巢组织进行RNA测序 (RNA-seq) 分析.

主要成果:

Lvit-GIH2编码了一个前体,一个信号和一个成熟的,与II型CHH子家族具有很高的相似性.
Lvit-GIH2被广泛表达,表达水平从雄性阶段增加到euhermaphrodite阶段.
Lvit-GIH2显著抑制了Lvit-Vg在肝胰腺和Lvit-VgR在卵巢中的表达.
RNA-seq显示,rGIH2负面调节卵巢通路,包括氨酸和氨酸代谢和亡,以及17bHSD8,IGFR和CHH等关键基因.

结论:

在卵巢中,Lvit-GIH2在Lysmata vittata的发育中起着显著的抑制作用.
这些发现表明,Lvit-GIH2充当淋巴腺抑制激素,通过分子信号通路影响生殖过程.

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