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通过利用水性/液晶界面来快速诊断活类沙门氏菌Typhimurium的新兴试验

Atiyeh Mehrzad¹, Asma Verdian², Mahboobe Sarabi-Jamab³

¹Department of Food Safety and Quality Control, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran; Department of Food Microbiology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.

Journal of hazardous materials

|August 1, 2024

概括

一个超敏感的胃感应阵列可以快速检测活的沙门氏菌 (Salmonella typhimurium) 细胞. 这种新的方法使用液晶和体来识别病原体,这对于预防食物传播疾病爆发至关重要.

科学领域:

生物感应是一种生物感应.
纳米技术纳米技术
食品安全食品安全

背景情况:

食物感染对公众健康构成重大风险.
快速准确地检测活病原体对于减轻疫情的爆发至关重要.
现有的方法往往需要先进行丰富或提取DNA,从而延迟结果.

研究的目的:

开发一种基于图像的超灵敏感应感应阵列,用于直接检测活的沙门氏菌 (Salmonella typhimurium) 细胞.
为了利用液晶 (LC) 和体为一种新的检测机制.
为病原体检测提供快速,准确和便携式平台.

主要方法:

开发一种使用表面活性剂装饰液晶 (LCs) 和aptamers (aptST) 的apt-sensing阵列.
使用LCs在水性/LC接口上的方向变化作为信号指示器.
监测aptST在与活生生的S. typhimurium结合后的形状变化,用于信号调制.

主要成果:

适应传感器显示,仅使用aptST就有信号关闭反应,在aptST相互作用时转换为信号启动.
观察到与S. typhimurium度相关的可逆信号关闭反应.
在大约30分钟内实现了1-1.1 × 10^6 CFU/mL的线性检测范围,低检测极限为1.2 CFU/mL.
在高回收率 (92.61-104.61%) 的增牛奶样本中得到验证的结果.

结论:

开发的口味传感器提供了一个快速和超敏感的方法来检测活的沙门氏菌 typhimurium.
便携式传感平台对现场检测各种食源性病原体充满希望.
这项技术可以显著提高食品安全和公共卫生监督.

关键词:

一个适应传感器.水性界面 - 水性界面液晶水晶是一种液晶.这是一个光学传感器.沙门氏菌的型号是沙门氏菌.

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