细胞膜作为用于加速骨修复的生物功能材料.
Emi Hatano1, Nahid Akhter2, Risa Anada3
1Advanced Research Center for Oral and Craniofacial Sciences Dental School, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Acta biomaterialia
|August 1, 2024
概括
血膜纳米片 (PMNFs) 通过吸引关键细胞到愈合部位来加速骨的修复. 这种无细胞疗法促进了骨的整合和硬度,与原生骨相当.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 血膜含有细胞相互作用和组织修复的重要组成部分.
- 之前已经证明了等离子体膜纳米片 (PMNFs) 能够核化骨形成.
- 优化PMNF生成和应用对于治疗用途至关重要.
研究的目的:
- 优化PMNFs的生成,分离和应用,作为骨缺陷修复的无细胞疗法.
- 为了评估PMNFs在加速骨愈合中的有效性,在小鼠形缺陷模型中.
- 将PMNF性能与其他骨移植材料进行比较.
主要方法:
- 从各种小鼠细胞系 (红细胞,骨质母细胞,纤维母细胞) 中分离出PMNFs,进行了预条件化和冷化.
- 在小鼠 (n=75) 中,PMNF被移植到临界大小的形缺陷中.
- 骨修复,细胞透,骨整合和机械性能使用组织学,体外和微硬性测试进行了评估.
主要成果:
- 冠状细胞衍生的PMNFs通过招募巨细胞,内皮细胞和骨质母细胞,在2周内显著加速骨修复.
- 在实验室中,PMNFs增强了细胞粘附,并且与无形酸盐 (ACP) 相比,显示出更好的骨整合.
- 6周后,再生骨表现出与原生骨相似的维克斯微硬度.
结论:
- 来自细胞膜的纳米碎片代表了有希望的无细胞生物材料用于骨再生.
- PMNFs具有多方面的生物功能特性,促进加速和整合的骨愈合.
- 这项研究确定PMNFs是加速骨缺陷修复的新疗法策略.
更多相关视频
09:35Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
9.7K
09:49Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
Published on: February 23, 2024
1.7K
相关概念视频
01:18The Bone Matrix
3.0K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
3.0K
01:40Bone Remodeling
38.2K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.2K
01:29The Extracellular Matrix
8.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
8.9K
01:22Enlargement of the Plasma Membrane
1.9K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
1.9K
01:24What are Membranes?
13.1K
A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries...
13.1K
01:27Fractures: Bone Repair
3.1K
Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the...
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the...
3.1K