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从埃及两次疫情中分离出来的口热病毒的序列和遗传学分析

Emad Al-Ebshahy¹, Ramy E El-Ansary², Jie Zhang³

¹Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Abees10th, Alexandria 21944, Egypt.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

|August 1, 2024

概括

埃及的口病 (FMD) 疫情显示出新出现的FMDV菌株,A和SAT2血清型. 基因分析揭示了与当前疫苗菌株的变异,需要更新牛的疫苗接种策略.

科学领域:

兽医病毒学兽医病毒学
流行病学流行病学
分子生物学分子生物学

背景情况:

口病 (FMD) 对埃及的畜牧业构成重大经济威胁,尽管采取了控制措施,但疫情仍在不断爆发.
在2018年和2022年期间,在贝希拉和卡弗尔-埃尔-谢赫省份报告了疫苗接种牛群中临床怀疑的口炎病例.

研究的目的:

检测和表征口病病毒 (FMDV) 菌株,涉及埃及最近爆发的疫情.
分析FMDV分离的遗传多样性和遗传学关系.
通过比较现场隔离物与当前疫苗菌株来评估潜在的疫苗不匹配.

主要方法:

实时RT-PCR使用泛FMDV原料检测表皮组织样本中的FMDVRNA.
部分VP1基因测序用于选定FMDV分离物的遗传学分析.
推断的氨基酸序列与疫苗菌株的比较分析.

主要成果:

在45%的检测样本中检测到FMDVRNA.
疫情涉及FMDV血清型A和SAT2 (2018年) 和专门的血清型A (2022年).
遗传学分析将SAT2分离物置于Lib-12谱系 (第七种拓型) 中,A血清型分离物置于亚洲和非洲拓型 (第四种基因型) 中.
与疫苗菌株相比,实地分离的VP1蛋白中观察到显著的氨基酸变异,特别是在抗原性位点.

结论:

埃及新出现的口腔疾病病毒株表现出基因多样性和与流通的疫苗菌株的变异.
目前的疫苗接种策略可能需要重新评估和更新,以确保对流行的FMDV野外菌株的有效保护.
进一步验证疫苗对新发现的FMDV基因型的疗效,对于疾病控制至关重要.

关键词:

埃及埃及埃及埃及在 FMD 和 FMD 的情况下,人类遗传学分析血清型类型血清型类型

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