甘维康提取物通过激活Nrf2/HO-1和MAPKs通路来保护肝细胞免受氧化损伤
Linxuan Xiao1, Mingming Zhao1, Ke-Gang Linghu1
1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
Fitoterapia
|August 1, 2024
概括
甘威康片 (GWK) 通过减少活性氧物种和改善细胞活力,保护肝细胞免受氧化损伤. 这种传统中医药激活了Nrf2/HO-1和MAPKs通路,为肝损伤提供了潜在的治疗策略.
科学领域:
- 传统中国医药 传统中国医药
- 肝病学 肝病学是一种肝病学.
- 分子生物学分子生物学
背景情况:
- 甘威康片 (GWK) 是一种用于肝脏疾病的传统中医药.
- 目前尚不清楚GWK的肝保护作用背后的确切机制.
- 了解GWK的作用对于其在肝脏保护中的临床应用至关重要.
研究的目的:
- 分析GWK的化学组成.
- 在实验室中研究GWK对氧化应激的肝脏保护作用.
- 阐明涉及GWK治疗作用的分子途径.
主要方法:
- 高性能液体染色学 (HPLC) 用于组合分析.
- 在体外研究使用H2O2诱导的氧化损伤在HL-7702肝细胞.
- 测量反应性氧物种 (ROS),马隆迪甲 (MDA),ALT和AST水平.
- 对Nrf2,HO-1,NQO1,KEAP1和MAPK通路蛋白质表达的分析.
主要成果:
- 量化了GWK的七个关键组成部分.
- GWK剂量依赖性保护肝细胞免受H2O2诱导的亡.
- GWK降低了细胞内ROS,MDA水平和ALT/AST活动.
- GWK上调了Nrf2/HO-1通路的组件,并激活了MAPKs (JUN,ERK,p38).
结论:
- GWK 显示出针对急性氧化损伤的显著肝保护作用.
- GWK的治疗作用包括激活Nrf2/HO-1信号通路.
- 此外,GWK还调节MAPKs通路,为其在肝细胞中的保护作用做出贡献.
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