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相关概念视频

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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Regulation of Expression Occurs at Multiple Steps02:24

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Negative Regulator Molecules01:23

Negative Regulator Molecules

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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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在衰老细胞中选择性蛋白质表达的基于RNA的逻辑.

Ward Jacobs1, Masoomeh Khalifeh1, Merijn Koot1

  • 1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen 6525 GA, the Netherlands.

The international journal of biochemistry & cell biology
|August 1, 2024
PubMed
概括

研究人员开发了一个mRNA逻辑电路来检测和消除衰老细胞. 该系统针对特定的microRNA签名,实现精确的细胞衰老检测和去除潜在的治疗应用.

关键词:
细胞衰老 细胞衰老这就是米RNA.纳米医学是一种纳米医学.治疗性的mRNA.

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞衰老,一种不可逆转的增长停止状态,有助于与年龄相关的疾病.
  • 选择性去除衰老细胞在癌症和纤维化中显示出治疗潜力.
  • 在体内有效检测衰老细胞对于了解它们的作用和治疗效果至关重要.

研究的目的:

  • 开发一种检测和选择性去除衰老细胞的方法.
  • 为了设计一个mRNA逻辑电路响应衰老特定的microRNA签名.
  • 为了验证这个系统对治疗干预的有用性.

主要方法:

  • 在人类初级纤维细胞中诱导和验证辐射诱导的衰老.
  • 使用RT-qPCR识别衰老期间上下调节的微RNA (miRNAs).
  • 一个mRNA逻辑电路的设计,其中包含miRNA结合点,用于衰老特异性蛋白质表达.

主要成果:

  • 证明了EGFP (增强绿色光蛋白) 的衰老特异表达,用于衰老细胞检测.
  • 为了选择性细胞去除,实现了构成性活性caspase-3的衰老特异性表达.
  • 验证了mRNA逻辑电路的功能,以响应与衰老相关的miRNA配置文件.

结论:

  • 开发的mRNA逻辑电路为向衰老细胞提供了一种新的策略.
  • 这种方法可以根据miRNA签名检测和选择性消除衰老细胞.
  • 为基于mRNA的治疗方案铺平了道路,治疗衰老细胞相关的疾病.