在嗅觉感官神经元剥离后,嗅球内部神经元的细胞类型和层特异性可塑性
Tenzin Kunkhyen1, Taryn R Brechbill1, Sarah P R Berg1
1Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA, 15213, USA.
Scientific reports
|August 1, 2024
概括
甲基马 (MMZ) 消去嗅觉感官神经元,但发生再生. 嗅觉球在这种再生过程中显示了细胞类型特定的内部神经元变化,揭示了可塑性机制.
科学领域:
- 神经科学是一个神经科学.
- 嗅觉系统可塑性 嗅觉系统的可塑性
- 哺乳动物的神经发生.
背景情况:
- 鼠标的嗅觉系统表现出独特的终身神经发生,允许从嗅觉上皮 (OE) 干细胞中再生嗅觉感官神经元 (OSN).
- 腹腔下区域也产生神经芽细胞,这些细胞迁移到嗅球 (OB) 形成抑制性内部神经元.
- 甲基马 (MMZ) 选择性地消去OSN,使得研究OB电路对传感输入损失和再生的反应成为可能.
研究的目的:
- 研究OSN损失和再生对小鼠嗅觉球 (OB) 内的不同内部神经元群体的影响.
- 在MMZ诱导的OSN切除后,对OB内部神经元的细胞类型和层特异性改变的特征.
主要方法:
- 小鼠接受了梅西马 (MMZ) 治疗,以诱导嗅觉感官神经元 (OSN) 的选择性切除.
- 免疫组织化学被用来量化各种内部神经元群体的密度和形态 (多巴胺基,表达帕瓦尔胺,表达卡莱丁宁) 在OB在治疗后的不同时间点 (7,14,35天).
- 评估了神经发生率,以确定它们对观察到的变化的贡献.
主要成果:
- 多巴氨基神经元密度在MMZ后7-14天下降,但在35天后恢复.
- 帕尔瓦胺表达性内部神经元密度保持不变,但在MMZ后7-14天, soma大小暂时减少,恢复35天.
- 在MMZ后7天,在特定的OB层中观察到卡列丁林表达神经元密度的短暂增加,不归因于神经发生的增加,但可能是表达的改变.
结论:
- 治疗MMZ诱导细胞类型和细胞层特定的变化,OB内部神经元密度和形态.
- 这些发现凸显了OB电路在响应传感输入损失和再生时的可塑性.
- 这项研究为在嗅觉系统恢复过程中OB内部的补偿机制提供了新的见解.
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