酸盐涂层的铁酸盐纳米颗粒增强了Rhodotorula toruloides胡卜素的生产
Nayra Ochoa-Viñals1, Dania Alonso-Estrada1, Rodolfo Ramos-González2
1Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
Bioprocess and biosystems engineering
|August 1, 2024
概括
酸盐涂层的磁纳米粒子 (MnFe2O4-CS) 增强了Rhodotorula toruloides酵母中的胡卜素生产. 这种纳米生物技术为增加代谢物产量和有效的生物质分离提供了一个有希望的方法.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 微生物学 微生物学
背景情况:
- 罗多托鲁拉 (Rhodotorula toruloides) 是一种酵母,以生产有价值的胡卜素而闻名.
- 磁纳米粒子为生物应用提供独特的特性.
- 优化胡卜素生产和生物质回收对于工业应用至关重要.
研究的目的:
- 为了研究Rhodotorula toruloides和磁性纳米粒子 (MnFe2O4和MnFe2O4-CS) 之间的相互作用.
- 为了评估这些纳米颗粒对R. toruloides中的胡卜素产生的影响.
- 探索磁纳米粒子在细胞固定和生物质分离方面的潜力.
主要方法:
- 通过共同沉和热水处理合成铁酸盐 (MnFe2O4) 和酸盐涂层 (MnFe2O4-CS) 纳米粒子.
- 使用XRD,磁力测量,DLS和FTIR进行纳米颗粒的表征.
- 细胞固定运动的动力学研究,吸附异热模拟和细胞活力评估 (Trypan蓝色试验).
主要成果:
- 基托桑涂层改变了纳米粒子大小和磁性.
- 最佳的细胞静止平衡时间为2小时.
- 胡卜素的产量显著增加至256.2μg/g干质量,MnFe2O4-CS在2.0 mg/mL时显著增加.
- 在与纳米粒子相互作用后,R. toruloides的活力仍然很高.
结论:
- 酸盐涂层的磁性纳米粒子 (MnFe2O4-CS) 能够有效地刺激R. toruloides中的胡卜素的产生.
- 酵母生物质的磁性分离是一种可行的和高效的技术.
- 这种纳米生物技术方法为增强酵母代谢物生产和回收提供了一个有希望的战略.
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