对中国新型突变性伪狂犬病病毒XJ5的重组和演变的分析
Luyao Jiang1,2,3,4, Jinlong Cheng1,2, Hao Pan1,2,3,4
1College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
BMC genomics
|August 1, 2024
概括
高致病性伪狂热病毒 (PRV) 菌株在中国造成了重大经济损失. 一种新分离的突变菌株XJ5显示出与最近的中国PRV分离物有很强的遗传联系,这表明了自然突变和重组模式.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 动物疾病控制和控制
背景情况:
- 伪狂犬病病毒 (PRV) 对中国的猪业造成了巨大的经济损害,自2011年以来,反复爆发的疫情归因于高度致病性,抗原性变异菌株.
- 这些变种菌株的出现对该地区的疾病控制和根除工作构成了重大挑战.
研究的目的:
- 来自中国的新孤立的高度致病性伪狂犬病病毒菌株 (XJ5) 的遗传特征和进化起源的研究.
- 分析潜在的突变和重组模式,有助于XJ5菌株的致病性增加.
主要方法:
- 孤立的伪狂犬病病毒菌株XJ5的全基因组测序 (基因银行加入号:OP512542).
- 对XJ5与以前报告的PRV菌株进行比较基因组分析和全基因组对齐,特别是那些在中国和全球分离出来的.
- 突变和重组分析以确定XJ5菌株的遗传变异和潜在起源.
主要成果:
- 2015年分离的XJ5菌株与原始分离物相比,具有显著更高的致病性.
- 全基因组分析显示,在2012年后在中国分离的XJ5和PRV菌株之间存在强烈的遗传关系,超过95%的同源性.
- 与非亚洲PRV菌株的同源性低于94%,表明中国分离品特有的潜在区域突变和重组事件.
- 自2011年以来,中国的病毒性PRV菌株似乎已经演变为与外国分离物分离的不同类型,可能是由于免疫不当和与疫苗菌株重组等因素.
结论:
- XJ5菌株很可能是中国流行突变PRV菌株的血统内自然突变的结果.
- 这些发现强调了持续基因组监测的重要性,以了解中国PRV的演变,并为有效的控制策略提供信息.
- 遗传分析支持了区域进化和重组事件的假设,这些事件塑造了中国病原性PRV菌株的现状.
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