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探索Saccharomyces cerevisiae中Pdr1p突变所赋予的2-乙醇应激反应机制

Huili Xia¹, Na Song², Daoqi Liu¹

¹College of Biological and Food Engineering, Huanghuai University, Zhumadian, 463000, Henan, China.

Biotechnology for biofuels and bioproducts

|August 1, 2024

概括

Pdr1p突变通过促进埃里希路径和改变氨基酸代谢来增强Saccharomyces cerevisiae中的2-phenylethanol (2-PE) 生产. 囊被认为是改善2-PE应激耐受性的关键氨基酸.

科学领域:

生物技术是生物技术.
微生物生理学微生物生理学
代谢工程是代谢工程.

背景情况:

2-乙醇 (2-PE) 的生产依赖于酵母耐受性,这种耐受性可以通过Pdr1p突变得到改善.
在Saccharomyces cerevisiae中Pdr1p介导的2-PE耐受性背后的分子机制在很大程度上是未知的.

研究的目的:

为了阐明Saccharomyces cerevisiae中Pdr1p介导的2-PE应激耐受性的分子机制.
确定关键的代谢途径和生物标志物,涉及到增强的2-PE耐受性.

主要方法:

对PDR1_862和5D酵母菌株的生理和生化分析.
转录组分析以确定在2-PE压力下基因表达的变化.
量化自由氨基酸含量,特别是氨酸.

主要成果:

Pdr1p突变使2-PE产量增加了16%,并且在3.5g/L的2-PE时,乙醇度降低了21%.
转录组分析显示了埃里希路径的上调,以及硫代谢和单碳池的变化.
自由氨基酸含量减少了31%,其中囊被确定为耐受性至关重要的氨基酸.

结论:

在S. cerevisiae中,Pdr1p突变通过上调Ehrlich通路来增强2-PE的产生.
Pdr1p突变调节细胞内氨基酸水平,其中氨酸可能作为2-PE应激耐受性的生物标志物.
这些发现提供了关于Pdr1p突变所赋予的2-PE应力耐受性的分子基础的见解.

关键词:

这种植物是Saccharomyces cerevisiae.2 - 乙乙醇应激压力是什么发生Pdr1p突变

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