人类肝瘤衍生生长因子的结合行为在SMYD1上
Jan-Kai Wu1,2,3, Ying-Ying Lee1,2,3, Hsin Hung1,2,3
1Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
The journal of physical chemistry. B
|August 2, 2024
概括
人类肝瘤衍生生长因子 (HDGF) 和其PWWP域与SMYD1DNA结合,C140域增强亲和力. 这项研究揭示了HDGFF.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 人类肝瘤衍生生长因子 (HDGF) 与细胞过程和癌症有关.
- HDGF 含有一个与染色体相关的 N-终端的 林-三-三-二 (PWWP) 域.
- 已知HDGF可以结合SMYD1促进体区域.
研究的目的:
- 研究HDGF的特定DNA结合行为,其PWWP域和C140域.
- 用蛋白质诱导的光变化来描述HDGF变体和SMYD1促进体之间的相互作用.
- 确定PWWP和C140域在HDGF的DNA结合亲和性和特异性中的作用.
主要方法:
- 利用蛋白质诱导的光变化技术.
- 使用Cy3标记的15bpSMYD1双链DNA (dsDNA).
- 分析了结合动力学,并使用结合模型确定了解离平衡常数.
主要成果:
- HDGF及其域与标记为Cy3的SMYD1 dsDNA的结合导致光强度显著下降 (灭).
- 与全长HDGF相比,PWWP域的SMYD1结合亲和力约为10倍弱.
- PWWP域对于DNA结合至关重要,而C140域增强了结合亲和力,并调节了序列特异性.
结论:
- HDGF,它的PWWP域和C140域与SMYD1 dsDNA.DNA相互作用.
- PWWP域对DNA结合至关重要,C140域调节这种相互作用.
- HDGF的C140域在特定序列的SMYD1.1结合中起着调节作用.
更多相关视频
13:34A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
Published on: April 6, 2016
10.2K
09:29Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
Published on: September 30, 2016
13.7K
相关概念视频
01:16TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
02:38Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K