在健康的产后养殖母马中进行马类疹病毒1 (EHV-1) 的分子检测
Luis G Arroyo1, Diego E Gomez1, Alison Moore1
1Departments of Clinical Studies (Arroyo, Gomez) and Pathobiology (Papapetrou, Lillie), Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1; Ontario Ministry of Agriculture, Food and Rural Affairs, 5th Floor NW, 1 Stone Road West, Guelph, Ontario N1G 4Y2 (Moore).
概括
马类疹病毒1 (EHV-1) 在发育后立即在健康的马匹中没有被检测到. 这表明,从堕胎的马匹的样本中检测到EHV-1表明了EHV-1-相关的堕胎.
科学领域:
- 兽医病毒学 兽医病毒学
- 马类传染病 马类传染病
背景情况:
- 马性疹病毒1 (EHV-1) 是马中的一个重要病原体.
- EHV-1可以引起呼吸系统疾病,堕胎和神经疾病.
研究的目的:
- 在健康的产后马匹中调查EHV-1核酸的存在.
- 为了确定EHV-1是否可以在胎后立即检测到鼻,阴道,全血或胎盘组织.
主要方法:
- 在产后24小时内从74只健康的产后马收集了296个样本 (鼻子拭子,阴道拭子,EDTA血液,胎盘组织).
- 利用聚合酶连锁反应 (PCR) 检测EHV-1的神经病原和非神经病原菌株.
主要成果:
- 所有296个样本的EHV-1核酸检测结果均为阴性.
- 没有证据表明EHV-1感染被发现在健康的马马在分娩后的直接期间.
结论:
- 在健康的产后母马中没有EHV-1的存在表明,在堕胎母马或胎儿膜中检测到的EHV-1表明了EHV-1相关的堕胎.
- 这一发现有助于区分不复杂的仔和EHV-1相关的并发症.
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