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在16HBE细胞中,SHP-1调解了香烟烟雾提取物诱导的上皮质-介质细胞转化和炎症

Quan He¹, Shuanglan Xu², Xiaomei Ma¹

¹Department of Respiratory and Critical Care Medicine, Zhenjiang Hospital of Integrated Traditional Chinese and Western Medicine, Zhenjiang, Jiangsu, 212000, China.

Open medicine (Warsaw, Poland)

|August 2, 2024

概括

在COPD中,Src-homology区域2域含酶1 (SHP-1) 的下调. 过度表达SHP-1可以保护肺细胞免受香烟烟雾提取物引起的损伤和炎症,这表明它在COPD中起着治疗作用.

科学领域:

肺部医学肺部医学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

含有Src-homology区域2域的酸酶1 (SHP-1) 已因其抗炎性质而闻名.
尚不清楚SHP-1在慢性阻塞性肺病 (COPD) 病原发生中的特定作用.

研究的目的:

在COPD模型中研究SHP-1的功能.
探索SHP-1作为COPD治疗点的潜力.

主要方法:

通过使用香烟烟雾提取物 (CSE) 刺激的16HBE细胞建立了体外COPD模型.
在16HBE细胞中过度表达SHP-1.
评估了细胞活力,细胞亡,迁移,上皮细胞-介质细胞过渡 (EMT) 和促炎性细胞因子的产生.
分析了P65和PI3K/AKT信号通路的激活.

主要成果:

在COPD患者和CSE治疗的16HBE细胞中,发现SHP-1表达减少.
过度表达SHP-1增强了细胞活力,并减少了CSE诱导的亡.
SHP-1过度表达逆转了CSE诱导的细胞迁移,EMT和促炎因子释放.
通过SHP-1过度表达,CSE诱导的P65和PI3K/AKT通路的激活被抑制.

结论:

SHP-1在CSE诱导的肺细胞损伤和炎症方面起着保护作用.
SHP-1减轻了CSE诱导的EMT和16HBE细胞的炎症,可能是通过抑制PI3K/AKT通路.
SHP-1可能是管理COPD发展和进展的潜在治疗点.

关键词:

慢性慢性肺炎是一种慢性慢性肺炎,COPD是一种慢性肺炎.在CSE中,CSE是CSE.在EMT中,EMT是EMT.通过PI3K/AKT路径.在SHP-1中使用.这是一种炎症炎症炎症炎症.

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