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Updated: Jun 18, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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限制单细胞蛋白质组学中蛋白质泄漏的影响

Andrew Leduc1, Yanxin Xu2, Gergana Shipkovenska2

  • 1Departments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, Northeastern University, Boston, MA 02115, USA.

bioRxiv : the preprint server for biology
|August 2, 2024
PubMed
概括

在单细胞蛋白质组学样本准备过程中最大限度地减少蛋白质泄漏对于高质量的数据至关重要. 这项研究确定了蛋白质泄漏特征,以准确分类受损细胞,提高实验可靠性.

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Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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