淋巴和静脉光滑肌肉的转录,发育和功能并行
Guillermo Arroyo-Ataz1, Alejandra Carrasco Yagüe1, Julia C Breda2
1Department of Pathology & Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, Massachusetts 02118, USA.
淋巴肌细胞 (LMCs) 与静脉光滑肌细胞 (SMCs) 共享基因表达,但具有不同的发育起源. 这项研究阐明了它们独特的收缩性质和潜在的祖先关系.
科学领域:
- 血管生物学 血管生物学
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 淋巴肌细胞 (LMC) 对于淋巴血管收缩至关重要,它们的功能障碍与淋巴有关.
- 对于LMC收缩性质的分子和发育基础的理解仍然不够充分.
研究的目的:
- 研究淋巴肌细胞 (LMC) 的转录和发育起源.
- 为了澄清LMCs的混合心肌细胞和血液血管光滑肌细胞 (SMC) 特性.
主要方法:
- 单细胞RNA测序 (scRNAseq) 的小鼠后肢淋巴和血管.
- 使用遗传标记 (Myh11,WT1,Nkx2.5) 的血统追踪研究.
- 淋巴和血管收缩性的体内成像.
主要成果:
- 轻微肌肉细胞和静脉肌肉细胞具有转录基因的相似性,与动脉状肌肉细胞不同.
- 乳腺癌并不是源于Myh11+光滑肌的祖先.
- 一个LMC和SMC的子集起源于WT1+间皮原体,但LMC不是来自Nkx2.5+心肌细胞原体.
- 动脉SMCs,而不是LMCs,在本研究中表达与心脏相关的基因.
结论:
- 静脉性SMC和LMC可能来自相关的间皮原始体.
- 轻微肌肉细胞和静脉SMC采用类似的基因表达程序来进行收缩功能.
- 脊髓瘤的发育和功能与心肌细胞和动脉瘤SMC血统有所区别.
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