高吞吐量方法用于同时选膜透性和毒性,以发现新的冷保护剂
Nima Ahmadkhani1, James D Benson2, Ali Eroglu3
1School of Chemical, Biological and Environmental Engineering, Oregon State University.
bioRxiv : the preprint server for biology
|August 2, 2024
概括
研究人员开发了一种更快的方法来选冷保护剂 (CPAs) 进行器官玻璃化. 这种高通量方法评估了CPA毒性和细胞膜透性,确定了更安全,更有效的冷保存选择.
科学领域:
- 生物技术和生物医学工程 生物技术和生物医学工程
- 低温生物学和冷保存方法
背景情况:
- 玻璃化是器官和组织的关键冷保存技术.
- 需要高度的细胞透型冷保护剂 (CPA),这带来了毒性挑战.
- 有限的CPA选择阻碍了有效的器官玻璃化.
研究的目的:
- 开发一种高通量选方法来评估CPA属性.
- 为了加快细胞膜透性和CPA毒性的测量.
- 确定具有有利的毒性和透性概况的新型CPAs用于冷保存.
主要方法:
- 开发了一种用于快速测量细胞膜透性的高通量试验.
- 在96井板格式内对CPA毒性的综合同时评估.
- 在4°C和室温下测试了27个CPA (5个常见,22个不常见).
主要成果:
- 实现透度测量速度比传统方法快100倍.
- 确定了23种CPA,具有良好的毒性和透性特征.
- 证明了该方法在选大量潜在CPA的有效性.
结论:
- 开发的高通量方法显著提高了CPA的选.
- 这种方法有助于识别用于器官玻璃化新型,毒性较低的CPA.
- 该方法是推进冷保存技术的有希望的工具.
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