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在酵母溶酶样真空体中的V-ATPase分解是复制性衰老中溶酶功能障碍的表型驱动因素

Fiza Hashmi¹, Patricia M Kane¹

¹Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY USA.

bioRxiv : the preprint server for biology

|August 2, 2024

概括

随着年龄的增长,由于V-ATPase分解,溶解体酸性减弱. 卡路里限制和维护RAVE复合体水平可以防止这种情况,保护细胞功能并延长酵母的寿命.

科学领域:

细胞生物学细胞生物学
衰老研究研究衰老研究
分子机制的分子机制

背景情况:

在不同物种中,随着年龄的增长,溶酶体酸化和功能下降.
V-ATPases对于有机细胞酸化至关重要,它们的活性通过可逆分解来调节.
酵母菌 (Saccharomyces cerevisiae) 作为研究细胞衰老的模型.

研究的目的:

调查V-ATPase分解在酵母复制老化中的作用.
探索卡路里限制对衰老过程中V-ATPase功能的影响.
为了确定调节V-ATPase组装和拆卸在衰老细胞中的因素.

主要方法:

使用酵母*Saccharomyces cerevisiae*作为一个复制老化模型.
在老化细胞中观察到V-ATPase分解为V1和V0亚复合体.
评估了热量限制和基因操纵 (RAVE复合物,Oxr1) 对V-ATPase和真空 pH 的影响.

主要成果:

在老化的酵母细胞 (≥5个分裂) 中,V-ATPases分解,导致真空的化.
热量限制可以防止V-ATPase分解,并保持老细胞中真空 pH 恒温.
RAVE复合物促进组装,而Oxr1促进拆卸;这些因素的突变会影响寿命. 拉维2水平随着年龄的增长而下降,其过度表达延迟了拆卸.

结论:

减少V-ATPase组合有助于与年龄相关的溶酶体酸化损失.
V-ATPase分解是酵母复制寿命的一个关键因素.
准V-ATPase组合可能为抵消与年龄相关的细胞功能障碍提供策略.

关键词:

衰老的衰老衰老的衰老这种植物是Saccharomyces cerevisiae.热量限制热量限制溶解体是溶解体的组成部分.质子的质子.

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