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解码RNA代谢通过RNA相关的CRISPR查在人类细胞中的代谢

Patrick J Nugent^1,2, Heungwon Park¹, Cynthia L Wladyka³

¹Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle WA, USA.

bioRxiv : the preprint server for biology

|August 2, 2024

概括

研究人员开发了一种新的CRISPR查方法 (ReLiC),以识别RNA代谢的基因调节者. 这种方法揭示了基因网络如何控制RNA拼接,翻译和衰变,影响细胞生长和疾病.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生化学

背景情况:

人类细胞利用数千种与RNA相关的蛋白质进行RNA代谢.
大多数RNA代谢事件的完整调节器集在很大程度上是未知的.
了解这些调节剂对于理解细胞功能和疾病至关重要.

研究的目的:

开发一种大规模的并行查方法,以全面识别RNA代谢的调节者.
研究控制RNA拼接,翻译和衰变的基因网络之间的相互作用.
探索细胞生长和mRNA翻译之间的合,并确定药物点.

主要方法:

开发和应用一种与RNA相关的CRISPR (ReLiC) 查方法.
对编码RNA相关蛋白质的2092个人类基因进行了淘汰选.
整合与多体体的生物化学分离和化学基因组概况.

主要成果:

ReLiC屏幕揭示了基因网络中的模块化相互作用,这些基因网络控制着mRNA拼接,翻译和衰变.
确定了生长适应性和mRNA转化之间的路径特定合.
捕获了内子保留和外子跳转的差异调节,并且破译了mRNA衰变的上游翻译调节者.

结论:

ReLiC是一个多功能平台,用于发现和剖析人类RNA代谢的调节原理.
该研究发现了对RNA代谢过程复杂调节的新见解.
这些发现对了解细胞对药物和疾病状态的反应有影响.

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相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...