在动物中建模酒精消费,使用两瓶选择的家庭饮酒和可选的基于液量计的微结构分析
Shikun Hou1, Nathaly M Arce Soto1, Elizabeth J Glover1
1Center for Alcohol Research in Epigenetics, Department of Psychiatry, University of Illinois Chicago, Chicago, IL, USA.
bioRxiv : the preprint server for biology
|August 2, 2024
概括
研究人员现在可以使用高分辨率的液计系统来研究大鼠的乙醇消耗. 这种LIQ HD系统的调整允许并行详细分析饮酒模式和摄入水平.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 动物行为 动物行为
背景情况:
- 两瓶选择的家用饮是一种用于研究动物乙醇消耗的标准方法.
- 传统方法提供有限的数据分辨率,每天只测量一次摄入量.
- 先进的lickometer系统提供高分辨率,一数据,但通常是针对小鼠的.
研究的目的:
- 审查标准和配备液位计的两瓶选择家庭饮用程序.
- 引入适应开源LIQ HD度仪系统在老鼠中使用的方法.
- 为了使多个老鼠的乙醇摄入模式能够进行并行,高分辨率的分析.
主要方法:
- 审查已建立的两瓶选择家庭饮用协议.
- 对LIQ HD系统的硬件修改用于老鼠 (较大的).
- 开发了一种3D打印的瓶盖,与标准的老鼠水瓶相兼容.
主要成果:
- 成功地适应了LIQ HD度仪系统用于大鼠.
- 能够同时对多种动物的乙醇摄入量进行高分辨率测量.
- 为研究人员提供灵活性,可以在标准或配备液位计的设置之间进行选择.
结论:
- 经过调整的LIQ HD系统为研究大鼠乙醇消耗提供了价格实惠和用户友好的解决方案.
- 这种方法显著提高了临床前研究中酒精摄入量研究的数据分辨率.
- 方便对酒精饮酒行为背后的神经生物学机制进行详细的审讯.
相关概念视频
Mass Spectrometry: Alcohol Fragmentation
Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the intensity of the molecular ion peak in the mass spectrum is weak or sometimes absent. The fragmentation patterns for alcohols occur in two ways, i.e. ⍺-cleavage and dehydration. During ⍺-cleavage, the bond at the ⍺-position adjacent to the hydroxyl group cleaves to give a resonance-stabilized cation and a radical. However, intramolecular dehydration...
Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...


