提高生物工程根再生的临床安全性:使用没有动物成分的介质
Yuzhen Zhan1,2,3, Aizhuo Qian1,2,3, Jieya Gao4
1College of Stomatology, Chongqing Medical University, Chongqing, China.
Heliyon
|August 2, 2024
概括
无动物成分 (ACF) 介质支持生物工程根再生,为动物血清提供更安全的临床替代品. 这种方法成功地在体内再生了牙组织,证明了它在牙组织工程方面的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
- 牙科研究 牙科研究
背景情况:
- 生物工程根再生的临床应用受到与含有动物血清的介质相关的伦理和安全问题所阻碍.
- 开发无动物成分 (ACF) 的介质对于推进牙组织工程的临床可行性至关重要.
研究的目的:
- 评估ACF介质作为生物工程根再生的更安全替代品的有效性.
- 为了比较在ACF介质培养的人类牙纤维干细胞 (hDPSCs) 与含血清介质 (SCM) 的生物特性和再生能力.
主要方法:
- 在实验室中使用ACF介质或SCM在三维 (3D) 人工处理牙矩阵 (hTDM) 中培养hDPSC.
- 通过定量实时PCR (qPCR) 和西方斑点检测评估了牙生成潜力.
- 将hDPSCs/hTDM复合体移植到裸体小鼠体内,通过组织学分析评估体内再生.
主要成果:
- ACF介质增强了hDPSCs的迁移,但最初稍微抑制了增殖,而不影响细胞衰老或细胞亡.
- 经过7天的3D培养,ACF介质在hDPSCs中促进了关键牙基因 (DMP1,DSPP,OCN,RUNX2) 和β-素III的高表达.
- 在体内,ACF介质支持牙,牙肉和牙周带状组织的再生,与SCM相似.
结论:
- ACF介质是生物工程根再生的可行替代方案.
- 使用ACF介质可以提高牙组织工程策略的临床安全性,以进行根部再生.
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