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依赖光的循环节律控制O-GlcNAc循环影响成年斑马鱼的认知功能

Jiwon Park¹, Dong Yeol Kim¹, Eok-Soo Oh²

¹Department of Biomedical Science, Program in Biomedical Science and Engineering, Department of Physiology and Biophysics, College of Medicine, Inha University, Incheon, Korea.

Journal of pineal research

|August 2, 2024

概括

大脑中的蛋白质O-GlcNAcylation节奏对于昼夜循环和记忆至关重要. 破坏这些节奏会损害学习和记忆,突出的是O-GlcNAcylation.

科学领域:

神经科学是一个神经科学.
时间生物学时间生物学
分子生物学分子生物学

背景情况:

昼夜循环调节许多生理过程,包括大脑功能.
蛋白质O-GlcNAcylation是一种涉及细胞调节的翻译后修饰.

研究的目的:

研究斑马鱼大脑中24小时蛋白质O-GlcNAcylation节奏的作用.
确定昼夜干扰对O-GlcNAcylation的影响及其与神经元功能,学习和记忆的关系.

主要方法:

斑马鱼模型被用来研究昼夜节律,通过逆转光暗周期和给予黑素.
分析了蛋白质O-GlcNAcylation,O-GlcNAc转移酶 (OGT) 和O-GlcNAcase (OGA) 的表达.
评估了使用OSMI-1的OGT抑制对行为和基因表达的影响.

主要成果:

循环节律扰乱损害了O-GlcNAcylation节奏,并在斑马鱼大脑中改变了OGT/OGA表达,显著影响了学习和记忆.
昼夜循环影响了组织素H2B的O-GlcNAcylation,与H3三甲基化变化相关,这些变化被循环改变破坏.
OSMI-1治疗破坏了清醒-睡眠模式,并抑制了关键的突触可塑性基因 (c-fos,bdnf,calm1),减少了O-GlcNAcylated H2B和OGT与基因促进剂的结合.

结论:

O-GlcNAc 基因代码的循环循环参与调节突触可塑性和大脑功能.
蛋白质O-GlcNAcylation作为一个关键的翻译后机制,将昼夜信号与神经功能相结合,以控制生理节律.

关键词:

昼夜循环周期的循环周期.六胺生物合成途径基斯顿基因基因基因基因基因基因基因基因基因基因基因基因基因基因斑马鱼是一种斑马鱼.

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