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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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相关实验视频

Updated: Jun 18, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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在高通量质谱学中采用样本特定的MS/MS方法

David M Cox1, Xuejiao Yin2, Jahangir Alam3

  • 1SCIEX, Concord, Ontario L4K 4V8, Canada.

Journal of the American Society for Mass Spectrometry
|August 2, 2024
PubMed
概括
此摘要是机器生成的。

本研究介绍了高通量质谱 (HT-MS) 的实时样本状态集成,以实现样本特定的数据采集. 这一创新克服了HT-MS中的方法设定挑战,以加速药物发现.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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科学领域:

  • 分析化学 分析化学
  • 质谱测量质量谱测量
  • 药物发现 药物发现 药物发现

背景情况:

  • 高通量质谱 (HT-MS) 对于加速药物发现至关重要,因为它能够快速分析大量样本集.
  • 当前的HT-MS平台面临的挑战是,在连续数据流中的单个样本中应用特定的数据采集方法.
  • 无染色技术的HT-MS技术可以提高样本读取速度,但需要先进的数据处理策略.

研究的目的:

  • 开发一种新的方法,在HT-MS平台上实时激活特定样本的MS/MS数据采集方法.
  • 为了应对在持续数据采集中方法设置的挑战,以进行高通量分析.
  • 提高HT-MS的可用性和稳定性,用于药物发现和其他高吞吐量应用.

主要方法:

  • 集成实时样本加载状态检测.
  • 开发一个自动化系统来触发样本特定的MS/MS数据采集方法.
  • 在高通量音响弹射质谱平台上实施.
  • 通过多重反应监测 (MRM),高分辨率MRM (MRMHR) 和依赖信息的获取模式进行验证.

主要成果:

  • 基于实时样本状态的有效方法切换.
  • 在各种数据采集窗口持续时间中实现了高信号可重现性.
  • 在不同的MS/MS获取模式 (MRM,HR,IDA) 中验证了该方法.
  • 证实了开发的解决方案的用户友好和强大的性质.

结论:

  • 这种新的方法成功地整合了实时样本状态,使HT-MS中的动态方法切换成为可能.
  • 这一进步简化了HT-MS的方法设置,提高了效率和可重复性.
  • 该解决方案扩大了HT-MS平台在药物发现和其他高通量分析领域的适用性.